在患有卵巢囊肿疾病的奶牛卵巢中，转化生长因子β受体1（TGFBR1）、转化生长因子β受体2（TGFBR2）、转化生长因子β受体3（TGFBR3）、激活素受体1B（ACVR1B）和激活素受体2B（ACVR2B）的表达发生改变。
Expression of TGFBR1, TGFBR2, TGFBR3, ACVR1B and ACVR2B is altered in ovaries of cows with cystic ovarian disease.
作者信息
Matiller Valentina, Hein Gustavo J, Stassi Antonela F, Angeli Emmanuel, Belotti Eduardo M, Ortega Hugo H, Rey Florencia, Salvetti Natalia R
机构信息
Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL)/Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Esperanza, Santa Fe, Argentina.
Facultad de Ciencias Veterinarias del Litoral, UNL, Esperanza, Santa Fe, Argentina.
出版信息
Reprod Domest Anim. 2019 Jan;54(1):46-54. doi: 10.1111/rda.13312.
The objective of this study was to examine the expression of transforming growth factor beta receptor (TGFBR)1, TGFBR2, TGFBR3, activin receptor (ACVR)1B and ACVR2B in ovaries of cows with cystic ovarian disease (COD). The expression of the selected receptors was determined by immunohistochemistry in sections of ovaries from cows with ACTH-induced and spontaneous COD. Expression of TGFBR1 and TGFBR3 was higher in granulosa cells of cysts from cows with spontaneous COD than in tertiary follicles from the control group. Additionally, TGFBR3 expression was higher in granulosa cells of cysts from cows with ACTH-induced COD than in those from the control group and lower in theca cells of spontaneous and ACTH-induced cysts than in tertiary control follicles. There were no changes in the expression of TGFBR2. ACVR1B expression was higher in granulosa cells of tertiary follicles of cows with spontaneous COD than in the control group, whereas ACVR2B expression was higher in cysts of the spontaneous COD group than in tertiary follicles from the control group. The alterations here detected, together with the altered expression of the ligands previously reported, indicate alterations in the response of the ligands in the target cells, modifying their actions at cellular level.
本研究的目的是检测转化生长因子β受体（TGFBR）1、TGFBR2、TGFBR3、激活素受体（ACVR）1B和ACVR2B在患有囊性卵巢疾病（COD）的奶牛卵巢中的表达。通过免疫组织化学方法，在患有促肾上腺皮质激素（ACTH）诱导型和自发性COD的奶牛卵巢切片中测定所选受体的表达。自发性COD奶牛囊肿颗粒细胞中TGFBR1和TGFBR3的表达高于对照组的三级卵泡。此外，ACTH诱导型COD奶牛囊肿颗粒细胞中TGFBR3的表达高于对照组，而自发性和ACTH诱导型囊肿的卵泡膜细胞中TGFBR3的表达低于三级对照卵泡。TGFBR2的表达没有变化。自发性COD奶牛三级卵泡颗粒细胞中ACVR1B的表达高于对照组，而自发性COD组囊肿中ACVR2B的表达高于对照组的三级卵泡。此处检测到的变化，以及先前报道的配体表达改变，表明靶细胞中配体反应发生改变，从而在细胞水平上改变了它们的作用。
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