Department of Biochemistry, University of Kerala, Thiruvananthapuram, Kerala 695581, India.
J Ethnopharmacol. 2018 Dec 5;227:8-17. doi: 10.1016/j.jep.2018.08.022. Epub 2018 Aug 16.
Hibiscus rosa sinensis petals possess wide range of pharmacological properties, with remarkable nutritional values. Diabetes is one of the most devastating diseases affecting the world today. A few side effects associated with the use of insulin and oral hypoglycemic agents prompted us to search new bioactive principles from antidiabetic plants used in traditional medicine.
The anti-diabetic therapeutic potential of the flavonoids rich ethyl acetate fraction of Hibiscus rosa sinensis petals (EHRS) was evaluated.
High glucose (25 mM) induced apoptotic model of diabetes in RIN-m5F pancreatic β-cells was used for the study.
EHRS elevated the release of insulin in pancreatic cells and modulated apoptotic signaling cascades. It significantly reduced NF-κB nuclear translocation, thereby down-regulated the expressions of major inflammatory cytokines and up-regulated expressions of pancreatic β-cell functional genes such as, foxO-1, Ucn-3, Pdx-1, MafA and Nkx6.1. On comparison with its constituent phytochemicals, superior protective effect shown by EHRS may be due to the additive action of these phytoconstituents.
Results of the present study suggest hibiscus petals as a natural source and functional food of potential therapeutics to protect pancreatic β-cells in experimental diabetes mellitus.
木槿花瓣具有广泛的药理学特性，具有显著的营养价值。糖尿病是当今影响世界最严重的疾病之一。一些与胰岛素和口服降糖药相关的副作用促使我们从传统医学中用于治疗糖尿病的植物中寻找新的生物活性成分。
评估富含类黄酮的木槿花瓣乙酸乙酯部分（EHRS）的抗糖尿病治疗潜力。
在 RIN-m5F 胰腺β细胞中，使用高葡萄糖（25mM）诱导的糖尿病细胞凋亡模型进行研究。
EHRS 可提高胰腺细胞中胰岛素的释放，并调节细胞凋亡信号通路。它显著降低了 NF-κB 的核易位，从而下调了主要炎症细胞因子的表达，并上调了胰腺β细胞功能基因的表达，如 foxO-1、Ucn-3、Pdx-1、MafA 和 Nkx6.1。与其中的植物化学成分相比，EHRS 表现出更好的保护作用，这可能是由于这些植物成分的协同作用。
本研究结果表明，木槿花瓣是一种天然来源和功能性食品，具有治疗实验性糖尿病的潜力，可以保护胰腺β细胞。
