用于对抗生物膜形成的精油的制备型高效液相色谱馏分。

Preparative HPLC fraction of essential oil against biofilm forming .

作者信息

Yang Xinjun, Rajivgandhi Govindan Nadar, Ramachandran Govindan, Alharbi Naiyf S, Kadaikunnan Shine, Khaled Jamal M, Almanaa Taghreed N, Manoharan Natesan, Viji Rajan

机构信息

Department of Pediatrics, 3201 Hospital, No. 783, Tianhan Avenue, Hantai District, Hanzhong, Shaanxi 723000, China.

Medical Microbiology and Marine Pharmacology Laboratory, Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu 620024, India.

出版信息

Saudi J Biol Sci. 2020 Oct;27(10):2853-2862. doi: 10.1016/j.sjbs.2020.07.008. Epub 2020 Jul 10.

Recent years () biofilm formation (BF) is emerging thread worldwide. For tackling this problem, we have chosen Hibiscus rosa-. pneumoniae. The HPLC purified essential oils (EOs sinensis (H. rosa-sinensis) (HRS) to inhibit the BF K) of H. rosa-sinensis was performed against BF and showed concentration dependent biofilm inhibition. At the MBIC of EOs (90 µg/ml), the biofilm inhibition was showed at 92% against selected BF . The biofilm metabolic assay, exopolysaccharide quantification and hydrophobicity index variation results exhibited with 88%, 92% and 89% reduction at 90 μg/mL was observed respectively. In addition, the morphological modification of MBIC treated was clearly viewed by scanning electron microscope (SEM). Overall, all the invitro experiments result were confirmed that the MBIC of H. rosa-sinensis EOs was very effective against BF

近年来，生物膜形成（BF）在全球范围内已成为一个新出现的问题。为了解决这个问题，我们选择了扶桑。对扶桑（Hibiscus rosa-sinensis）（HRS）的高效液相色谱纯化精油（EOs）进行了抗BF实验，结果显示其具有浓度依赖性的生物膜抑制作用。在EOs的最低生物膜抑制浓度（MBIC）（90μg/ml）下，对选定的BF的生物膜抑制率为92%。生物膜代谢分析、胞外多糖定量和疏水性指数变化结果分别显示，在90μg/mL时，其降低率分别为88%、92%和89%。此外，通过扫描电子显微镜（SEM）可以清楚地观察到经MBIC处理后的形态变化。总体而言，所有体外实验结果均证实，扶桑EOs的MBIC对BF非常有效