Department of Food Science, UNESP - São Paulo State University, Araraquara, Brazil.
Department of Food Science, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
Appl Microbiol Biotechnol. 2018 Oct;102(20):8827-8840. doi: 10.1007/s00253-018-9234-8. Epub 2018 Aug 18.
This study aimed to evaluate the effects of three treatments, i.e., Bifidobacterium longum BB-46 (T1), B. longum BB-46 combined with the pectin (T2), and harsh extracted pectin from lemon (T3) on obesity-related microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®). The effects of the treatments were assessed by the analysis of the intestinal microbial composition (using 16S rRNA gene amplicon sequencing) and the levels of short-chain fatty acids (SCFAs) and ammonium ions (NH). Treatments T2 and T3 stimulated members of the Ruminococcaceae and Succinivibrionaceae families, which were positively correlated with an increase in butyric and acetic acids. Proteolytic bacteria were reduced by the two treatments, concurrently with a decrease in NH. Treatment T1 stimulated the production of butyric acid in the simulated transverse and descending colon, reduction of NH as well as the growth of genera Lactobacillus, Megamonas, and members of Lachnospiracea. The results indicate that both B. longum BB-46 and pectin can modulate the obesity-related microbiota; however, when the pectin is combined with B. longum BB-46, the predominant effect of the pectin can be observed. This study showed that the citric pectin is able to stimulate butyrate-producing bacteria as well as genera related with anti-inflammatory effects. However, prospective clinical studies are necessary to evaluate the anti/pro-obesogenic and inflammatory effects of this pectin for future prevention of obesity.
本研究旨在利用人类肠道微生物模拟系统（SHIME®）评估三种处理方法，即长双歧杆菌 BB-46（T1）、长双歧杆菌 BB-46 与果胶的混合物（T2）和柠檬中提取的果胶（T3）对肥胖相关微生物群的影响。通过分析肠道微生物组成（使用 16S rRNA 基因扩增子测序）和短链脂肪酸（SCFA）和铵离子（NH）水平来评估处理效果。处理 T2 和 T3 刺激了瘤胃球菌科和琥珀酸弧菌科成员的生长，这与丁酸和乙酸的增加呈正相关。两种处理均减少了蛋白水解菌，同时 NH 减少。处理 T1 刺激了模拟横结肠和降结肠丁酸的产生，降低了 NH，并促进了乳杆菌属、巨单胞菌属和lachnospiracea 成员的生长。结果表明，长双歧杆菌 BB-46 和果胶都可以调节与肥胖相关的微生物群；然而，当果胶与长双歧杆菌 BB-46 结合使用时，可以观察到果胶的主要作用。本研究表明，柠檬酸果胶能够刺激产生丁酸的细菌以及与抗炎作用相关的属。然而，需要进行前瞻性临床研究来评估这种果胶的抗/促肥胖和抗炎作用，以便将来预防肥胖。
