Precision Livestock and Nutrition Unit, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, ULiège, Gembloux, Belgium.
Research Foundation for Industry and Agriculture, National Scientific Research Foundation (FRIA-FNRS), Brussels, Belgium.
J Sci Food Agric. 2019 Oct;99(13):5720-5733. doi: 10.1002/jsfa.9837. Epub 2019 Jul 31.
Dietary strategies such as the inclusion of prebiotics have been suggested for modulating intestinal microbiota. In piglets, this strategy could result in a reduction of post-weaning-associated disorders and the use of antibiotics. To date, mainly purified fractions have been tested for their prebiotic effects at weaning while trials of potential health-promoting effects of products and corresponding by-products remain rare. In this study, fructan- and pectin-based ingredients have been tested in a two-step in vitro model for their fermentation kinetics as well as for their short-chain fatty acid production and microbiota profiles in fermentation broth as indicators for their prebiotic activity.
Chicory root, in contrast to chicory pulp, exhibited an extensive and rapid fermentation similar to inulin and oligofructose, although butyrate levels of root and pulp did not reach those of the purified fractions. Chicory pulp showed higher relative levels of Lactobacillus spp., Bifidobacterium spp., Clostridium cluster IV and butyryl-CoA:acetate-CoA transferase gene abundance compared to chicory root. Sugar beet pulp, orange and citrus by-products displayed extensive gas fermentation patterns, equivalent to those of purified pectin, and revealed an elevated butyrate production compared to purified pectin. Moreover, several orange and citrus by-products displayed significantly higher relative levels of Bifidobacterium spp. in comparison to purified pectin.
Chicory root and pulp as well as orange and citrus by-products appear to be promising ingredients for piglet diets for modulating intestinal fermentation for health purposes. © 2019 Society of Chemical Industry.
膳食策略，如添加益生元，已被建议用于调节肠道微生物群。在仔猪中，这种策略可能会减少断奶相关疾病和抗生素的使用。迄今为止，主要是在断奶时测试了具有益生元作用的纯分数，而关于潜在促进健康的产品和相应副产物的试验仍然很少。在这项研究中，以菊苣根和果胶为基础的成分在两步体外模型中进行了测试，以评估其发酵动力学以及发酵液中短链脂肪酸的产生和微生物群落谱，作为其益生元活性的指标。
与菊苣浆相比，菊苣根表现出广泛而快速的发酵，类似于菊糖和低聚果糖，尽管根和浆的丁酸水平没有达到纯分数的水平。菊苣浆中乳杆菌属、双歧杆菌属、梭菌簇 IV 和丁酰 CoA：乙酸 CoA 转移酶基因丰度的相对水平高于菊苣根。糖甜菜浆、橙和柑橘副产品显示出广泛的气体发酵模式，与纯果胶相当，并显示出比纯果胶更高的丁酸产量。此外，与纯果胶相比，几种橙和柑橘副产品中双歧杆菌属的相对水平明显更高。
菊苣根和浆以及橙和柑橘副产品似乎是有希望的仔猪饲料成分，用于调节肠道发酵以促进健康。 © 2019 化学工业协会。
