Cui W X, Xu W, Yang Q W, Li Y R, Hu R, Cheng L Y
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018 Aug 7;53(8):592-596. doi: 10.3760/cma.j.issn.1673-0860.2018.08.007.
To investigate the expression of marker proteins in vocal cord leukoplakia, and to find markers for the early stage of diagnosis and prognosis of precancerous lesions. The study included 119 cases, 68 cases of vocal cord leukoplakia (22 cases with epithelial simple hyperplasia, 46 cases with epithelial dysplasia), and 51 cases of vocal cords benign lesions(31 cases of vocal cord polyps, 20 cases of Reinke's edema). The expression of p53, Ki-67, p21, Survivin, p16, p27, PTEN, c-Myc and vascular endothelial growth factor (VEGF) in vocal cords leukoplakia were detected, vocal cord benign lesions (vocal cord polyps and Reinke's edema) acted as controls, comparing the expression differences of different pathological tissue. Data was analyzed by SPSS 22.0 software. The expression of p53, p16, Ki-67, VEGF in vocal cord benign lesions and vocal cords leukoplakia with epithelial simple hyperplasia did not show significant differences. There was a grading increase in the positive expression of p53, Ki-67 in the vocal cord leukoplakia with epithelial dysplasia contrasting to those in vocal cord benign lesions and vocal cords leukoplakia with epithelial simple hyperplasia (p53: χ(2)=13.340, =0.002, Ki-67: χ(2)=53.386, =0.000). The expression of p27, PTEN, c-Myc in vocal cord benign lesions and vocal cords leukoplakia with epithelial dysplasia did not show significant differences. There was a grading increase in the positive expression of p21 Survivin in vocal cords leukoplakia with epithelial dysplasia contrasting to those in vocal cord benign lesions (<0.05). Expression of Survivin in vocal cords leukoplakia with mild-moderate epithelial dysplasia showed a significant increase than those in vocal cord benign lesions (<0.05). The positive expression grade of p21 showed a rising trend (=0.073) between the different grades of dysplasia. The positive expression grade of p53, Ki-67, p21 Survivin showed an increase in vocal cords leukoplakia with epithelial dysplasia contrasting to those in vocal cord benign lesions, which might be an implication for evaluating the diagnosis and prognosis of precancerous lesions. Expression of p21 was correlated to the degrees of dysplasia and expression of Survivin showed a significant difference in early stage of epithelial dysplasia contrasting to benign lesions.
探讨声带白斑中标志物蛋白的表达情况，寻找癌前病变早期诊断及预后的标志物。本研究纳入119例患者，其中声带白斑68例（上皮单纯增生22例，上皮发育异常46例），声带良性病变51例（声带息肉31例，任克氏水肿20例）。检测声带白斑中p53、Ki-67、p21、Survivin、p16、p27、PTEN、c-Myc及血管内皮生长因子（VEGF）的表达，以声带良性病变（声带息肉和任克氏水肿）作为对照，比较不同病理组织的表达差异。采用SPSS 22.0软件进行数据分析。声带良性病变与上皮单纯增生的声带白斑中p53、p16、Ki-67、VEGF的表达无显著差异。与声带良性病变及上皮单纯增生的声带白斑相比，上皮发育异常的声带白斑中p53、Ki-67的阳性表达呈分级增加（p53：χ(2)=13.340，P=0.002；Ki-67：χ(2)=53.386，P=0.000）。声带良性病变与上皮发育异常的声带白斑中p27、PTEN、c-Myc的表达无显著差异。与声带良性病变相比，上皮发育异常的声带白斑中p21、Survivin的阳性表达呈分级增加（P<0.05）。轻度-中度上皮发育异常的声带白斑中Survivin的表达较声带良性病变显著增加（P<0.05）。不同发育异常等级之间p21的阳性表达等级呈上升趋势（P=0.073）。与声带良性病变相比，上皮发育异常的声带白斑中p53、Ki-67、p21、Survivin的阳性表达增加，这可能对癌前病变的诊断及预后评估有一定意义。p21的表达与发育异常程度相关，Survivin的表达在上皮发育异常早期与良性病变相比有显著差异。
