Urano M, Iwata S, Takasu A, Mori S, Sakurai K, Katoh H, Yamamoto S, Kasahara M, Kuroda M, Hakuno T
Department of Otolaryngology, Fujita Health University, Aichi.
Nihon Jibiinkoka Gakkai Kaiho. 1996 Mar;99(3):395-401.
We investigated the proliferative activities in epithelial hyperplasia and dysplasia of the human vocal cords as precancerous lesions, using immunohistochemical staining with anti-PCNA and MIB-1 (anti-Ki-67) monoclonal antibody. The series for this study consisted of nine patients with hyperplasia, 12 with mild dysplasia, ten with moderate dysplasia, eight with severe dysplasia, eight with vocal cord polyps, and 14 with invasive squamous cell carcinoma. The following results were obtained: 1) The mean PCNA labeling index was 1.12 +/- 1.05 (MEAN +/- STD%) in polyp, 4.88 +/- 2.02 in hyperplasia, 2.76 +/- 1.76 in mild dysplasia, 3.80 +/- 2.03 in moderate dysplasia, 6.12 +/- 3.01 in severe dysplasia, and 19.07 +/- 10.37 in invasive cancer. 2) The mean MIB-1 positive rates were 5.50 +/- 2.47 (MEAN +/- STD%) in polyp, 13.08 +/- 6.86 in hyperplasia, 16.55 +/- 7.34 in mild dysplasia, 15.94 +/- 6.73 in moderate dysplasia, 21.43 +/- 8.16 in severe dysplasia, and 41.48 +/- 14.05 in invasive cancer. In cases of hyperplasia, dysplasia and invasive cancer, PCNA labeling index values and MIB-1 positive rates increased in proportion to the histological atypical grade increasing. Some lesions which recurred or progressed to cancer were found show high expression of PCNA and MIB-1. In cancer cases, there was no significant correlation between the PCNA labeling index, MIB-1 positive rates and either the degree of tumor cell differentiation or the T-classification. There was a positive correlation between the PCNA labeling index and MIB-1 positive rates among all cases. In this study, those cases showing a high PCNA labeling index and/or MIB-1 positive rates may indicate the possibility of recurrence or progression to malignancy in precancerous lesions of the vocal cords.
我们采用抗增殖细胞核抗原（PCNA）和抗MIB-1（抗Ki-67）单克隆抗体免疫组化染色法，研究了人声带上皮增生和发育异常这些癌前病变中的增殖活性。本研究系列包括9例增生患者、12例轻度发育异常患者、10例中度发育异常患者、8例重度发育异常患者、8例声带息肉患者和14例浸润性鳞状细胞癌患者。获得了以下结果：1）息肉中PCNA标记指数的平均值为1.12±1.05（平均值±标准差%），增生中为4.88±2.02，轻度发育异常中为2.76±1.76，中度发育异常中为3.80±2.03，重度发育异常中为6.12±3.01，浸润性癌中为19.07±10.37。2）息肉中MIB-1阳性率的平均值为5.50±2.47（平均值±标准差%），增生中为13.08±6.86，轻度发育异常中为16.55±7.34，中度发育异常中为15.94±6.73，重度发育异常中为21.43±8.16，浸润性癌中为41.48±14.05。在增生、发育异常和浸润性癌病例中，PCNA标记指数值和MIB-1阳性率随组织学非典型分级增加而升高。发现一些复发或进展为癌症的病变显示PCNA和MIB-1高表达。在癌症病例中，PCNA标记指数、MIB-1阳性率与肿瘤细胞分化程度或T分期之间均无显著相关性。所有病例中PCNA标记指数与MIB-1阳性率之间存在正相关。在本研究中，那些显示PCNA标记指数高和/或MIB-1阳性率高的病例可能提示声带癌前病变复发或进展为恶性肿瘤的可能性。
