Does restriction enzyme analysis reveal changes in herpes simplex virus DNA after latency in mouse ganglia?

Possible changes in the herpes simplex virus (HSV) genome after latent infection in mice were studied by restriction enzyme analysis. Lumbosacral ganglia of C 57 Bl mice, genitally infected with a low passage strain of HSV-1 were explanted 3-8 month post infectionem and cocultivated with human embryo fibroblasts. Four virus isolates reactivated from the ganglia were compared with the original virus inoculated into the mice. The cleavage patterns yielded by 7 restriction enzymes - i.e. Eco RI, Hind III, Bam HI, Kpn I, Xba I, Bg1 II, HpaI - did not indicate any differences.