Zhu Lingping, Shu Zhiqun, Sun Xiaoming
Department of General Practice, Shenzhen Longhua District Central Hospital, Shenzhen, People's Republic of China.
Department of General Practice, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China,
Cancer Manag Res. 2018 Aug 1;10:2337-2346. doi: 10.2147/CMAR.S168594. eCollection 2018.
The purpose of this study was to investigate the expression of different miRNAs in nonmetastatic and metastatic endometrial cancer Existing evidence indicates that there are many factors affecting the metastasis of endometrial cancer, and miRNAs play an unique role in many processes of endometiral cancer.
miRNA sequences were downloaded from The Cancer Genome Atlas Project database, and Bioinformatics technique was used to deal with those data.
We elucidated the relation between differentially expressed miRNAs and clinical information for a total of 260 tumor tissues and 22 tumor tissues that had metastasized. We used the threshold of <0.05| log 2 FC | >1.2 to identify potential miRNAs. Four differentially expressed miRNAs were identified in nonmetastatic and metastatic endometrial cancers. Further differential analysis of metastatic tissue revealed that miR-1247 is associated with metastasis of endometrial cancer to the lung, and miR-3200 is associated with the clinical stage of endometrial cancer. A functional enrichment analysis showed that the four miRNAs may be involved in multiple pathways of cancer, including the Wnt, NOTCH, and TGF-β signaling pathways and signaling pathways regulating pluripotency of stem cells. Protein-protein interaction analysis showed that , , , , , , , and are hub genes of relevant miRNAs in endometrial cancers.
The current study indicates that these four miRNAs may be related to molecular markers of metastasis of endometrial cancer.
本研究旨在调查不同miRNA在非转移性和转移性子宫内膜癌中的表达情况。现有证据表明，有许多因素影响子宫内膜癌的转移，且miRNA在子宫内膜癌的许多过程中发挥独特作用。
从癌症基因组图谱计划数据库下载miRNA序列，并使用生物信息学技术处理这些数据。
我们阐明了总共260个肿瘤组织和22个已转移肿瘤组织中差异表达的miRNA与临床信息之间的关系。我们使用<0.05| log 2 FC |>1.2的阈值来鉴定潜在的miRNA。在非转移性和转移性子宫内膜癌中鉴定出4种差异表达的miRNA。对转移组织的进一步差异分析表明，miR-1247与子宫内膜癌向肺转移有关，miR-3200与子宫内膜癌的临床分期有关。功能富集分析表明，这4种miRNA可能参与癌症的多种途径，包括Wnt、NOTCH和TGF-β信号通路以及调节干细胞多能性的信号通路。蛋白质-蛋白质相互作用分析表明， 、 、 、 、 、 、 和 是子宫内膜癌中相关miRNA的枢纽基因。
当前研究表明，这4种miRNA可能与子宫内膜癌转移的分子标志物有关。
