Liu Kuanbo, Wang Fen, Wang Wenzhao, Dong Caihong
Guizhou Provincial Key Laboratory of Fermentation Engineering and Biological Pharmacy, Guizhou University, Guiyang, China.
School of Liquor-making and Food Engineering, Guizhou University, Guiyang, China.
Mycology. 2017 Sep 11;8(4):259-266. doi: 10.1080/21501203.2017.1375040. eCollection 2017.
-(2-Hydroxyethyl)-adenosine (HEA), which was the first calcium antagonist derived from biological sources, has been intensively investigated because of its ability to inhibit tumour cell proliferation, restrain inflammation, protect kidneys and function as a sedative and insecticide. Up to now, the production of HEA has been detected only in a few species such as and . Here, we found a new HEA-producing fungus, which was identified as based on morphological and phylogenetic characteristics. The HEA production was verified by reversed-phase high-performance liquid chromatography/mass spectrometry in this fungus. Moreover, HEA was also detected in the mycelia of two other strains from different origins, but not in the culture medium of all tested strains. The maximum production of HEA (0.8483 ± 0.0439 mg/g mycelia dry weight) was achieved on day 7. The fungal biomass and kinetics of HEA production exhibited similar trends over the duration of the culture period. This is the first report describing HEA production in , which suggests that this fungal strain may have new applications as a source of HEA.
（2-羟乙基）腺苷（HEA）是首个源自生物源的钙拮抗剂，因其具有抑制肿瘤细胞增殖、抑制炎症、保护肾脏以及具有镇静和杀虫作用而受到深入研究。到目前为止，仅在少数物种如[具体物种1]和[具体物种2]中检测到HEA的产生。在此，我们发现了一种新的产生HEA的真菌，基于形态学和系统发育特征将其鉴定为[真菌名称]。通过反相高效液相色谱/质谱法在该真菌中验证了HEA的产生。此外，在另外两株来自不同来源的[菌株名称]的菌丝体中也检测到了HEA，但在所有测试的[菌株名称]菌株的培养基中未检测到。在第7天实现了HEA的最大产量（0.8483±0.0439mg/g菌丝体干重）。在培养期间，真菌生物量和HEA产生动力学呈现出相似的趋势。这是首次报道描述[真菌名称]中HEA的产生，这表明该真菌菌株作为HEA的来源可能具有新的应用。
