Fu Hsin-I, Hsu Jui-Hsia, Li Tsung-Ju, Yeh Shu-Hsing, Chen Chin-Chu
Biotech Research Institute Grape King Bio Ltd Taoyuan City Taiwan.
Institute of Food Science and Technology National Taiwan University Taipei City Taiwan.
Food Sci Nutr. 2021 Jul 16;9(9):4905-4915. doi: 10.1002/fsn3.2440. eCollection 2021 Sep.
, an entomopathogenic fungus, is a source of traditional Chinese medicine in China. Due to the low yield of wild , artificial cultivation approaches will be needed to meet the increasing market demand. Using bioreactor culture can increase mass production and the abundance of the active component, N6-(2-hydroxyethyl)-adenosine (HEA). Here, we describe a safety assessment for a novel mycelium preparation method. Many studies have confirmed the safety of mycelia. However, the acute safety pharmacology of the enriched with the high HEA (3.90 mg/g) compound has not been evaluated. This study evaluated the central nervous system (CNS), cardiovascular system, and respiratory system in ICR male mice via oral gavage administration. For each requested item, two batches of eight mice tested on a vehicle (0.5% carboxymethyl cellulose, CMC) and mycelia (1,000 mg/kg) were performed. The heart rate at 60 min for the vehicle and mycelium treatment was 700.3 ± 55.4 and 603.0 ± 42.3 bpm, respectively ( = .4279). For echocardiographic analysis, the LV mass of the vehicle and drug treatment was 86.7 ± 6.4 and 80.2 ± 7.7, respectively ( = .0933). In the respiratory test, the tidal volume of the vehicle and drug treatments was 0.11 ± 0.01 and 0.14 ± 0.01 at 60 min, respectively ( = .4262). These results demonstrate that the oral administration of HEA-enriched mycelia is safe for the CNS, cardiovascular, and respiratory systems.
作为一种昆虫病原真菌，在中国是一种中药来源。由于野生产量低，需要采用人工栽培方法来满足不断增长的市场需求。使用生物反应器培养可以提高大规模生产和活性成分N6-(2-羟乙基)-腺苷（HEA）的丰度。在此，我们描述了一种新型菌丝体制备方法的安全性评估。许多研究已经证实了菌丝体的安全性。然而，富含高HEA（3.90mg/g）化合物的菌丝体的急性安全药理学尚未评估。本研究通过口服灌胃给药评估了ICR雄性小鼠的中枢神经系统（CNS）、心血管系统和呼吸系统。对于每个要求的项目，对两组各八只小鼠进行了测试，一组给予赋形剂（0.5%羧甲基纤维素，CMC），另一组给予菌丝体（1000mg/kg）。赋形剂和菌丝体处理组在60分钟时的心率分别为700.3±55.4和603.0±42.3次/分钟（P = 0.4279）。对于超声心动图分析，赋形剂组和药物处理组的左心室质量分别为86.7±6.4和80.2±7.7（P = 0.0933）。在呼吸测试中，赋形剂组和药物处理组在60分钟时的潮气量分别为0.11±0.01和0.14±0.01（P = 0.4262）。这些结果表明，口服富含HEA的菌丝体对中枢神经系统、心血管和呼吸系统是安全的。
