一种用于活细胞内源性过氧亚硝酸盐检测的快速响应和高特异性 Si-Rhodamine 探针。
A fast-response and highly specific Si-Rhodamine probe for endogenous peroxynitrite detection in living cells.
机构信息
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Functional Materials Chemistry, and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
出版信息
Org Biomol Chem. 2019 Feb 13;17(7):1875-1880. doi: 10.1039/c8ob01598h.
Peroxynitrite (ONOO-) is involved in a variety of physiological and pathological processes. We designed and synthesized a fluorescent probe SiNH based on Si-rhodamine. The nanoprobe SiNH encapsulated within the amphiphilic copolymer exhibited fast response within 10 s, and it was highly specific for ONOO- in aqueous solution. It is demonstrated that the nanoprobe SiNH is applicable for real-time tracing of endogenous ONOO- in living cells.
过氧亚硝酸盐（ONOO-）参与了多种生理和病理过程。我们设计并合成了一种基于 Si-罗丹明的荧光探针 SiNH。纳米探针 SiNH 被包裹在两亲共聚物中，在 10 秒内快速响应，并且对水溶液中的 ONOO-具有高度特异性。实验证明，纳米探针 SiNH 可用于实时追踪活细胞内的内源性 ONOO-。
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