Kuramitsu Madoka, Okuma Kazu, Nakashima Makoto, Sato Tomoo, Sasaki Daisuke, Hasegawa Hiroo, Umeki Kazumi, Kubota Ryuji, Sasada Keiko, Sobata Rieko, Matsumoto Chieko, Kaneko Noriaki, Tezuka Kenta, Matsuoka Sahoko, Utsunomiya Atae, Koh Ki-Ryang, Ogata Masao, Ishitsuka Kenji, Taki Mai, Nosaka Kisato, Uchimaru Kaoru, Iwanaga Masako, Sagara Yasuko, Yamano Yoshihisa, Okayama Akihiko, Miura Kiyonori, Satake Masahiro, Saito Shigeru, Watanabe Toshiki, Hamaguchi Isao
Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, Japan.
Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
Microbiol Immunol. 2018 Oct;62(10):673-676. doi: 10.1111/1348-0421.12644.
Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08-3.49 copies/100 cells. The maximum difference between laboratories was 3.2-fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.
人类嗜T淋巴细胞病毒1型（HTLV-1）前病毒的定量聚合酶链反应（qPCR）用于HTLV-1检测及评估HTLV-1相关疾病的风险。在本研究中，开发了一种用于标准化HTLV-1 qPCR的参考物质。制备了用Jurkat细胞稀释的冻干TL-Om1细胞，并通过数字PCR确定了前病毒载量（PVL）的指定值为2.71拷贝/100个细胞。九个日本实验室使用各自的方法评估该参考物质的PVL为1.08 - 3.49拷贝/100个细胞。各实验室之间的最大差异为3.2倍。通过使用包含该参考物质指定值的公式校正测得的PVL，应可最大程度减少此类差异。
