Department of General Chemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No.1, Bucharest 011061, Romania.
Department of Biomaterials, National Institute of Research and Development for Biological Sciences, Bioproducts, Splaiul Independentei, No.296, Bucharest 060031, Romania.
Mar Drugs. 2018 Aug 18;16(8):287. doi: 10.3390/md16080287.
In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg on the enzymatic activity and the antibacterial investigations while using Gram-negative and Gram-positive bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg. The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.
在本文中，我们介绍了新壳聚糖基膜的制备和表征，同时使用了一种新的生物技术来固定碱性磷酸酶（ALP）。该技术涉及金属离子的掺入，以开发新的生物聚合物载体。通过傅里叶变换红外光谱（FT-IR）和扫描电子显微镜技术（SEM）评估了所提出的膜的化学结构和形态特征。电感耦合等离子体质谱（ICP-MS）证明了金属离子随时间的释放。此外，还研究了 Mg 对酶活性和革兰氏阴性菌和革兰氏阳性菌的抗菌作用、溶血和生物相容性行为的影响。将 ALP 固定在壳聚糖膜组成中，然后掺入 Mg，与不含 Mg 的壳聚糖基膜相比，得到的聚合物载体具有更高的细胞活力。新的基于壳聚糖、ALP 和金属离子的生物聚合物载体在生物医学应用中具有良好的性能。
