Rato Ana, Joaquim Sandra, Tavares Tânia G, Martins Zita E, Guedes A Catarina, Pereira Luís F, Machado Jorge, Matias A Margarete, Gonçalves José F M, Vaz-Pires Paulo, Magnoni Leonardo J, Ozório Rodrigo O A, Matias Domitília
Interdisciplinary Centre of Marine Environmental Research (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal.
Department of Sea and Marine Resources, Portuguese Institute for Sea and Atmosphere (IPMA, I.P.), Av. 5 de Outubro s/n, 8700-305, Olhão, Portugal.
Biol Open. 2018 Oct 2;7(9):bio035923. doi: 10.1242/bio.035923.
The current study evaluated the microalgae replacement by dry macroalgae () in the reproductive success and biochemical composition of the Pacific oyster () during broodstock conditioning. Five nutritional regimes were tested: 100% macroalgae (diet 1), 50% macroalgae+50% microalgae (diet 2), 25% macroalgae+75% microalgae (diet 3) and 100% microalgae (diet 4). An unfed group was used as a negative control. The microalgae blend was composed of 33% and 67% diatoms (75% +25% ). Gonadal maturation was reflected in the physiological condition of the individuals. All treatments, except diet 1, showed an increase in condition index and were fully matured at the end of the trial, with the best physiological condition observed in oysters fed diet 3 and diet 4. Protein and total lipid content increased during the conditioning period, whereas glycogen content decreased. Oysters conditioned with diet 3 had higher protein and total lipid content and lower glycogen content than the other treatments. In addition, diet 3 showed the highest percentage of viable veliger larvae. The current study demonstrated that it is possible to replace 25% of microalgae with macroalgae in the broodstock conditioning, minimizing the operative cost in bivalve hatcheries.This article has an associated First Person interview with the first author of the paper.
本研究评估了在亲贝暂养期间，用干大型藻类（）替代微藻对太平洋牡蛎（）繁殖成功率和生化组成的影响。测试了五种营养方案：100%大型藻类（日粮1）、50%大型藻类+50%微藻（日粮2）、25%大型藻类+75%微藻（日粮3）和100%微藻（日粮4）。一个未喂食组用作阴性对照。微藻混合物由33%的 和67%的硅藻（75% +25% ）组成。性腺成熟反映在个体的生理状况上。除日粮1外，所有处理的状况指数均有所增加，并且在试验结束时均完全成熟，日粮3和日粮4喂养的牡蛎生理状况最佳。在暂养期间，蛋白质和总脂质含量增加，而糖原含量下降。与其他处理相比，日粮3喂养的牡蛎蛋白质和总脂质含量更高，糖原含量更低。此外，日粮3显示出可游动面盘幼虫的比例最高。本研究表明，在亲贝暂养过程中用大型藻类替代25%的微藻是可行的，这将使双壳贝类孵化场的运营成本降至最低。本文配有对该论文第一作者的第一人称访谈。
