Rato Ana, Pereira Luís F, Joaquim Sandra, Gomes Romina, Afonso Cláudia, Cardoso Carlos, Machado Jorge, Gonçalves José F M, Vaz-Pires Paulo, Magnoni Leonardo J, Matias Ana M, Matias Domitília, Bandarra Narcisa M, Ozório Rodrigo O A
Interdisciplinary Centre of Marine Environmental Research (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, Matosinhos, 4450-208, Portugal.
Department of Sea and Marine Resources, Portuguese Institute for Sea and Atmosphere (IPMA, I.P.), Av. 5 de Outubro s/n, Olhão, 8700-305, Portugal.
Lipids. 2019 Sep;54(9):531-542. doi: 10.1002/lipd.12177. Epub 2019 Jul 17.
The fatty acid (FA) profile of oysters generally reflects the dietary FA composition. Moreover, incorporation of FA into tissues is modulated by various metabolic factors, and final composition will depend upon the dietary sources, cumulative intake, and oysters' development stage. Thus, the aim of this study was to assess the impact of dietary incorporation of seaweed (SW) Ulva rigida, in replacement of traditional microalgae diet, on the FA composition of Pacific oysters Crassostrea gigas, during broodstock conditioning. The dietary conditioning consisted of direct replacement of microalgae (33% Tisochrysis lutea, 50.25% Skeletonema costatum, and 16.75% Chaetoceros calcitrans) by SW at four different substitution levels (0%, 25%, 50%, and 100% diet). The dietary docosahexaenoic acid (DHA) (22:6n-3) and eicosapentaenoic acid (EPA) (20:5n-3) contents showed a positive correlation with the dietary microalgae level. During the trial, oysters fed with higher percentages of microalgae revealed a depletion of DHA and accumulation of EPA. The 100% SW caused a significant reduction in oxygen consumption and, consequently, in the standard metabolic rate. Based on these results, a partial substitution of up to 25% of dietary microalgae seems to be a suitable alternative, because it elicited similar results to the commercial 100% microalgae diet.
牡蛎的脂肪酸（FA）谱通常反映了饮食中的FA组成。此外，FA掺入组织受到多种代谢因素的调节，最终组成将取决于饮食来源、累积摄入量和牡蛎的发育阶段。因此，本研究的目的是评估在亲鱼培育期间，用海藻（SW）硬石莼替代传统微藻饲料对太平洋牡蛎太平洋牡蛎FA组成的影响。饮食调节包括在四个不同替代水平（0%、25%、50%和100%饲料）下用SW直接替代微藻（33%的球等鞭金藻、50.25%的中肋骨条藻和16.75%的钙质角毛藻）。饮食中的二十二碳六烯酸（DHA）（22:6n-3）和二十碳五烯酸（EPA）（20:5n-3）含量与饮食中的微藻水平呈正相关。在试验期间，喂食较高比例微藻的牡蛎显示DHA减少，EPA积累。100%的SW导致耗氧量显著降低，因此标准代谢率也显著降低。基于这些结果，高达25%的饮食微藻部分替代似乎是一种合适的选择，因为它产生的结果与商业100%微藻饲料相似。
