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棘孢木霉菌株JLM45-3的一种新型双链RNA真菌病毒的分子特征

Molecular characterization of a novel double-stranded RNA mycovirus of Trichoderma asperellum strain JLM45-3.

作者信息

Zhang Tingting, Zeng Xiangxing, Cai Xiaoyao, Liu Hongmei, Zeng Zhu

机构信息

Engineering Research Center of Medical Biotechnology, Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, 550000, Guizhou, People's Republic of China.

Immune Cells and Antibody Engineering Research Center of Guizhou Province, Cell and Gene Engineering Innovative Research Groups of Guizhou Province, Guizhou Medical University, Guiyang, 550000, Guizhou, People's Republic of China.

出版信息

Arch Virol. 2018 Dec;163(12):3433-3437. doi: 10.1007/s00705-018-3988-2. Epub 2018 Aug 20.

Abstract

In this study, we describe a novel mycovirus isolated from Trichoderma asperellum, which was designated Trichoderma asperellum dsRNA Virus 1 (TaRV1). The sequence analysis revealed that TaRV1 has two discontinuous open reading frames (ORF), ORF1 and ORF2. A hypothetical protein and an RNA-dependent RNA polymerase are encoded by ORF1 and ORF2, respectively. Phylogenetic analysis based on RdRp sequences clearly places TaRV1 in a taxonomically unassigned dsRNA mycovirus group.

摘要

在本研究中,我们描述了一种从棘孢木霉中分离出的新型真菌病毒,将其命名为棘孢木霉双链RNA病毒1(TaRV1)。序列分析表明,TaRV1有两个不连续的开放阅读框(ORF),即ORF1和ORF2。ORF1和ORF2分别编码一种假定蛋白和一种RNA依赖性RNA聚合酶。基于RNA依赖性RNA聚合酶(RdRp)序列的系统发育分析清楚地将TaRV1置于一个分类学上未归属的双链RNA真菌病毒组中。

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