来自中国哈茨木霉的一种新型真菌病毒的全核苷酸序列。
Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China.
作者信息
Liu Chenchen, Li Mei, Redda Estifanos Tsegaye, Mei Jie, Zhang Jiantai, Elena Santiago F, Wu Beilei, Jiang Xiliang
机构信息
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Rd., Haidian District, Beijing, 100193, China.
Instituto de Biología Integrativa de Sistemas (CSIC-Universitat de València), Parc Cientific UV, Catedrático Agustín Escardino 9, 46980, Paterna, Valencia, Spain.
出版信息
Arch Virol. 2019 Apr;164(4):1213-1216. doi: 10.1007/s00705-019-04145-9. Epub 2019 Feb 12.
A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. The first segment was 2088 bp long and contained a single ORF (ORF1) encoding the RNA-dependent RNA polymerase (RdRP) (72.26 kDa). The second segment was 1634 bp long and also contained a single ORF (ORF2) encoding a hypothetical protein of 37.472 kDa. We named this novel mycovirus "Trichoderma harzianum bipartite mycovirus 1" (ThBMV1). Phylogenetic analysis showed that ThBMV1 clusters with other unclassified dsRNA mycoviruses.
在中国新疆分离得到的哈茨木霉137菌株中鉴定出一种新的真菌病毒。通过宏基因组测序、逆转录聚合酶链反应（RT-PCR）和RACE克隆确定了该真菌病毒的全基因组序列。该真菌病毒包含两个基因组片段。第一个片段长2088bp，包含一个单一开放阅读框（ORF1），编码依赖RNA的RNA聚合酶（RdRP）（72.26kDa）。第二个片段长1634bp，也包含一个单一开放阅读框（ORF2），编码一种假定的37.472kDa蛋白质。我们将这种新型真菌病毒命名为“哈茨木霉双分体真菌病毒1”（ThBMV1）。系统发育分析表明，ThBMV1与其他未分类的双链RNA真菌病毒聚类。
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