Biomedical Science, School of Diagnostic Sciences & Applied Health, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
Cardiovasc Toxicol. 2019 Feb;19(1):72-81. doi: 10.1007/s12012-018-9478-7.
Heart failure-associated morbidity and mortality is largely attributable to extensive and unregulated cardiac remodelling. Roselle (Hibiscus sabdariffa) calyces are enriched with natural polyphenols known for antioxidant and anti-hypertensive effects, yet its effects on early cardiac remodelling in post myocardial infarction (MI) setting are still unclear. Thus, the aim of this study was to investigate the actions of roselle extract on cardiac remodelling in rat model of MI. Male Wistar rats (200-300 g) were randomly allotted into three groups: Control, MI, and MI + Roselle. MI was induced with isoprenaline (ISO) (85 mg/kg, s.c) for two consecutive days followed by roselle treatment (100 mg/kg, orally) for 7 days. Isoprenaline administration showed changes in heart weight to body weight (HW/BW) ratio. MI was especially evident by the elevated cardiac injury marker, troponin-T, and histological observation. Upregulation of plasma levels and cardiac gene expression levels of inflammatory cytokines such as interleukin (IL)-6 and IL-10 was seen in MI rats. A relatively high percentage of fibrosis was observed in rat heart tissues with over-expression of collagen (Col)-1 and Col-3 genes following isoprenaline-induced MI. On top of that, cardiomyocyte areas were larger in heart tissues of MI rats with upregulation of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) gene expression, indicating cardiac hypertrophy. Interestingly, roselle supplementation attenuated elevation of plasma troponin-T, IL-6, IL10, and gene expression level of IL-10. Furthermore, reduction of cardiac fibrosis and hypertrophy were observed. In conclusion, roselle treatment was able to limit early cardiac remodelling in MI rat model by alleviating inflammation, fibrosis, and hypertrophy; hence, the potential application of roselle in early adjunctive treatment to prevent heart failure.
心力衰竭相关的发病率和死亡率在很大程度上归因于广泛和不受调节的心脏重塑。玫瑰茄（洛神花）花萼富含天然多酚，具有抗氧化和抗高血压作用，但它在心肌梗死后（MI）早期心脏重塑中的作用尚不清楚。因此，本研究旨在探讨玫瑰茄提取物对 MI 大鼠模型心脏重塑的作用。雄性 Wistar 大鼠（200-300g）随机分为三组：对照组、MI 组和 MI+玫瑰茄组。用异丙肾上腺素（ISO）（85mg/kg，皮下）连续两天诱导 MI，然后用玫瑰茄提取物（100mg/kg，口服）治疗 7 天。ISO 给药导致心脏重量与体重（HW/BW）比值发生变化。MI 尤其明显的是心脏损伤标志物肌钙蛋白-T 的升高和组织学观察。MI 大鼠的血浆水平和心脏基因表达水平的炎症细胞因子如白细胞介素（IL）-6 和 IL-10 上调。在 ISO 诱导的 MI 后，大鼠心脏组织中观察到胶原（Col）-1 和 Col-3 基因的过度表达，有相对较高百分比的纤维化。此外，MI 大鼠心脏组织中的心肌细胞面积较大，心房利钠肽（ANP）和脑利钠肽（BNP）基因表达上调，表明存在心肌肥大。有趣的是，玫瑰茄补充剂可减轻血浆肌钙蛋白-T、IL-6、IL10 和 IL-10 基因表达水平的升高。此外，还观察到心脏纤维化和肥大减少。总之，玫瑰茄治疗能够通过减轻炎症、纤维化和肥大来限制 MI 大鼠模型的早期心脏重塑，因此玫瑰茄在预防心力衰竭的早期辅助治疗中的潜在应用。
