Messa Mirko
Department of Neuroscience and Cell Biology, HHMI, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT, USA.
Methods Mol Biol. 2018;1847:13-22. doi: 10.1007/978-1-4939-8719-1_2.
More than a trillion nerve terminals interconnect neurons in the human brain. These terminals are fundamental for signal transmission and nerve cell communication. Among other techniques, the isolation of nerve terminals [or synaptosomes (Whittaker et al. Biochem J, 90(2):293-303, 1964)] has been fundamental to study the biochemistry and the physiology of the nervous system. This chapter describes the isolation and purification of intact synaptosomes from rodent brain tissue that can be used to further characterize synaptic structure and function and to examine the molecular mechanisms of neurotransmission.
超过一万亿个神经末梢在人类大脑中相互连接神经元。这些末梢对于信号传递和神经细胞通讯至关重要。在其他技术中，神经末梢（或突触体，惠特克等人，《生物化学杂志》，90(2):293 - 303，1964年）的分离对于研究神经系统的生物化学和生理学至关重要。本章描述了从啮齿动物脑组织中分离和纯化完整突触体的方法，这些突触体可用于进一步表征突触结构和功能，并研究神经传递的分子机制。
