从中枢神经系统分离线粒体。
Isolation of mitochondria from the CNS.
作者信息
Kristian Tibor
机构信息
Department of Anesthesiology, Organized Research Center, School of Medicine, University of Maryland, Baltimore, Maryland, USA.
出版信息
Curr Protoc Neurosci. 2010 Jul;Chapter 7:Unit 7.22. doi: 10.1002/0471142301.ns0722s52.
Abstract
This unit contains a protocol describing the isolation of brain mitochondria by using discontinuous Percoll gradient centrifugation. The Percoll density gradient centrifugation separates synaptosomes, myelin, and free nonsynaptic mitochondria released from cells during tissue homogenization into individual fractions. Mitochondria entrapped in synaptosomes (synaptic mitochondria) can be liberated using nitrogen cavitation and then further purified by Percoll gradient centrifugation. These methods yield mitochondria that exhibit good respiratory coupling and high respiratory rates.
摘要
本单元包含一个使用不连续Percoll梯度离心法分离脑线粒体的实验方案。Percoll密度梯度离心法可将组织匀浆过程中从细胞释放的突触体、髓磷脂和游离的非突触线粒体分离成单个组分。被困在突触体中的线粒体（突触线粒体）可通过氮气空化法释放，然后通过Percoll梯度离心法进一步纯化。这些方法所获得的线粒体具有良好的呼吸偶联和高呼吸速率。
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