Martin H, Voss K, Hufnagl P
Zentralbl Allg Pathol. 1986;131(2):137-42.
8 astrocytomas grade 1-4 and 2 glioblastomas were investigated by a system of automated microscope picture analysis. Histograms of nuclear area and shape from more than 1,000 measured nuclei per tumor were analysed. Both one- and two-dimensional histograms show findings which correlate with the malignant potential of the gliomas. The nuclear area rises with increasing tumor malignancy. On the other hand the measurements of nuclear shape factor are inversely related to the level of malignancy, indicating substantial irregularities in the nuclear structure. The 2D-histograms of both measured parameters also show changes which are correlated with the grade of tumor malignancy and which can be described as shift, loss of compactness and enlargement. The histogram method is discussed as an objectifiable process characterizing important cell and nuclear attributes connected with tumor malignancy. Histograms of both, nuclear area and shape can be helpful in the classification and grading of astrocytomas and glioblastomas.
通过自动显微镜图像分析系统对8例1 - 4级星形细胞瘤和2例胶质母细胞瘤进行了研究。分析了每个肿瘤1000多个测量细胞核的核面积和形状直方图。一维和二维直方图均显示出与胶质瘤恶性潜能相关的结果。核面积随肿瘤恶性程度的增加而增大。另一方面，核形状因子的测量值与恶性程度呈负相关，表明核结构存在明显不规则性。两个测量参数的二维直方图也显示出与肿瘤恶性程度相关的变化，可描述为偏移、致密性丧失和增大。讨论了直方图法作为一种可客观化的过程，用于表征与肿瘤恶性相关的重要细胞和核属性。核面积和形状的直方图有助于星形细胞瘤和胶质母细胞瘤的分类和分级。
