Indersmitten Tim, Berdyyeva Tamara, Aluisio Leah, Lovenberg Timothy, Bonaventure Pascal, Wyatt Ryan M
AnaBios Corporation, San Diego, California.
Janssen Research & Development, LLC, San Diego, California.
Curr Protoc Pharmacol. 2018 Sep;82(1):e42. doi: 10.1002/cpph.42. Epub 2018 Aug 20.
Imaging neuronal activity in awake behaving mice with miniature fluorescence microscopes requires the implementation of a variety of procedures. Surgeries are performed to gain access to the cell population of interest and to implant microscope components. After a recovery period, mice are trained to exhibit a desired behavior. Finally, neuronal activity is imaged and synchronized with that behavior. To take full advantage of the technology, selection of the calcium indicator and experimental design must be carefully considered. In this article, we explain the procedures and considerations that are critical for obtaining high-quality calcium imaging data. As an example, we describe how to utilize miniature fluorescence microscopy to image hippocampal place cell activity during linear track running in Thy1.GCaMP6f transgenic mice. © 2018 by John Wiley & Sons, Inc.
使用微型荧光显微镜对清醒行为小鼠的神经元活动进行成像需要实施多种程序。进行手术以接触到感兴趣的细胞群体并植入显微镜组件。经过恢复期后，训练小鼠表现出期望的行为。最后，对神经元活动进行成像并与该行为同步。为了充分利用该技术，必须仔细考虑钙指示剂的选择和实验设计。在本文中，我们解释了对于获得高质量钙成像数据至关重要的程序和注意事项。作为一个例子，我们描述了如何利用微型荧光显微镜对Thy1.GCaMP6f转基因小鼠在直线轨道奔跑期间的海马位置细胞活动进行成像。© 2018约翰威立父子公司版权所有
