Jacob Alexander D, Ramsaran Adam I, Mocle Andrew J, Tran Lina M, Yan Chen, Frankland Paul W, Josselyn Sheena A
Program in Neurosciences and Mental Health, Hospital for Sick Children, Toronto, Ontario, Canada.
Department of Psychology, University of Toronto, Toronto, Ontario, Canada.
Curr Protoc Neurosci. 2018 Jul;84(1):e51. doi: 10.1002/cpns.51. Epub 2018 Jun 26.
Miniaturized fluorescence microscopes for imaging calcium transients are a promising tool for investigating the relationship between behavior and population-level neuronal activity in rodents. However, commercially available miniature microscopes may be costly and, because they are closed source, may not be easily modified based on particular experimental requirements. Here, we describe how to build and use a low-cost compact head-mounted endoscope (CHEndoscope) system for in vivo calcium imaging. The CHEndoscope uses an implanted gradient index lens along with the genetically encoded calcium indicator GCaMP6 to image calcium transients from hundreds of neurons simultaneously in awake behaving mice. This system is affordable, open source, and flexible, permitting modification depending on the particular experiment. This article describes in detail the assembly, surgical implantation, data collection, and processing of calcium signals using the CHEndoscope system. The aim of this open framework is to provide an accessible set of miniaturized calcium imaging tools for the neuroscience research community. © 2018 by John Wiley & Sons, Inc.
用于成像钙瞬变的小型荧光显微镜是研究啮齿动物行为与群体水平神经元活动之间关系的一种很有前景的工具。然而，市售的微型显微镜可能成本高昂，而且由于它们是封闭源的，可能不容易根据特定的实验要求进行修改。在这里，我们描述了如何构建和使用一种低成本的紧凑型头戴式内窥镜（CHEndoscope）系统进行体内钙成像。CHEndoscope使用植入的梯度折射率透镜以及基因编码的钙指示剂GCaMP6，在清醒行为的小鼠中同时对数百个神经元的钙瞬变进行成像。该系统价格实惠、开源且灵活，可根据特定实验进行修改。本文详细描述了使用CHEndoscope系统进行钙信号的组装、手术植入、数据收集和处理。这个开放框架的目的是为神经科学研究界提供一套易于获取的小型钙成像工具。© 2018 John Wiley & Sons, Inc.
