Kunieda T, Ikadai H, Matsui M, Nomura N, Imamichi T, Ishizaki R
Imamichi Institute for Animal Reproduction, Saitama, Japan.
Biochem Genet. 1989 Oct;27(9-10):591-602. doi: 10.1007/BF02396154.
A novel restriction fragment length polymorphism in inbred rats was detected by Southern blot analysis with rat growth hormone cDNA as a probe. Four alleles, characterized by PstI fragments of 1.2, 1.1, 0.9, and 0.7 kb, respectively, were detected in 27 strains examined. The same distribution of polymorphisms was observed on digestion of DNAs of these strains with three other enzymes, PvuII, HindIII, and BamHI. Moreover, the same differences in length of allelic restriction fragments were obtained with these restriction enzymes as with PstI. These findings suggested that the polymorphism was caused by insertion or deletion of variable DNA segments in the second intron of the growth hormone gene. Linkage analyses using backcross progeny provided no evidence for close linkage between the restriction fragment length polymorphism locus and 10 other loci examined.
用大鼠生长激素cDNA作探针，通过Southern印迹分析在近交系大鼠中检测到一种新的限制性片段长度多态性。在所检测的27个品系中，检测到四个等位基因，其特征分别为PstI片段长度为1.2、1.1、0.9和0.7 kb。用另外三种酶PvuII、HindIII和BamHI消化这些品系的DNA时，观察到相同的多态性分布。此外，用这些限制性酶获得的等位基因限制性片段长度差异与用PstI获得的相同。这些发现表明，这种多态性是由生长激素基因第二内含子中可变DNA片段的插入或缺失引起的。利用回交后代进行的连锁分析没有提供限制性片段长度多态性位点与所检测的其他10个位点紧密连锁的证据。
