Gissel-Nielsen G
Ann Clin Res. 1986;18(1):61-4.
In 1963 Se-deficiency was observed for the first time in Denmark in a few sheep in West Jutland. The sheep were cured by injection of "Tokosel", and a survey of the Se-status of Danish fodder crops was initiated. A comprehensive set of data was produced during the early 70's, using the fluorometric method. The survey showed a general Se-deficiency in the whole country, and series of experiments were carried out to elucidate the possibility of raising the selenium level in plants from the native 0.02-0.04 ppm to more than the desired minimum of 0.05 ppm. Three different methods of application were tested: seed pretreatment, fertilizer enrichment, and foliar application. Seed pre-treatment has some disadvantages while the two other methods proved to be efficient and safe in a series of experiments and in tests on a large number of farms all over Denmark. These experiments and tests are discussed in detail. It is concluded that about 120 g Se/ha as sodium selenite, 10 g Se/ha as sodium selenate - both added through PK- or NPK-fertilizers, or foliar application of about 5 g Se/ha are sufficient yearly treatments to raise the native Se content of the Danish crops to levels of 0.05-0.1 ppm.
1963年，丹麦首次在日德兰半岛西部的几只绵羊身上发现了硒缺乏症。这些绵羊通过注射“托科塞尔”得以治愈，随后便启动了一项关于丹麦饲料作物硒状况的调查。在70年代早期，采用荧光分析法得出了一整套数据。调查显示，丹麦全国普遍存在硒缺乏的情况，于是开展了一系列实验，以阐明将植物中的硒含量从原本的0.02 - 0.04 ppm提高到超过理想最低值0.05 ppm 的可能性。测试了三种不同的施用方法：种子预处理、肥料富集和叶面喷施。种子预处理存在一些缺点，而在一系列实验以及丹麦各地众多农场进行的测试中证明，另外两种方法既有效又安全。详细讨论了这些实验和测试情况。得出的结论是，每年施用约120克/公顷的亚硒酸钠硒、10克/公顷的硒酸钠硒（均通过PK肥或NPK肥添加），或者叶面喷施约5克/公顷硒，足以将丹麦作物原本含有的硒含量提高到0.05 - 0.1 ppm的水平。
