Shutko A N, Shatinina N N
Ukr Biokhim Zh. 1977 Mar-Apr;49(2):42-4.
The levels of total DNA, RNA and protein synthesis were examined in control and in vivo irradiated thymocytes after their preincubation with exogenous DNA of different polymerization degree. The studies were made using the uridine-14C and lysine-14C. The influence of preliminary treatment of exogenous DNA or cells with actinomycin D (AMD) was studied as applied to the levels of their own and "induced" synthesis in the cells. It is shown that native exogenous DNA induced the increase in the synthesis of all three components only in the irradiated cells. The pretreatment of the irradiated cells or exogenous DNA with AMD completely inhibits the "induced" synthesis of RNA and protein while their own synthesis in suppressed only by 20%. Possible transcription and translation of highly polymerized exogenous DNA in the irradiated cells without its integration into the genome of the "host" is discussed.
在用不同聚合度的外源DNA预孵育后，检测对照及体内照射的胸腺细胞中总DNA、RNA和蛋白质合成水平。研究使用了尿苷-14C和赖氨酸-14C。研究了用放线菌素D（AMD）对外源DNA或细胞进行预处理对细胞自身及“诱导”合成水平的影响。结果表明，天然外源DNA仅在照射细胞中诱导了所有三种成分合成的增加。用AMD对照射细胞或外源DNA进行预处理完全抑制了RNA和蛋白质的“诱导”合成，而它们自身的合成仅被抑制20%。讨论了高聚合度外源DNA在照射细胞中可能的转录和翻译，而无需其整合到“宿主”基因组中。
