Zemmour J, Touraine J L, Devonec M, Royo C
Thymus. 1984;6(5):335-45.
Differentiation of spleen prothymocytes into Thy-1.2+ cells under the influence of thymosin requires transcription of DNA and translation of RNA, as demonstrated by the effects of inhibitors of DNA, RNA and protein synthesis. The lifespan of the induction message for this T cell differentiation appeared to be relatively short (30 to 60 min) in the presence of a reversible inhibitor of protein synthesis. When anisomycin, a protein synthesis inhibitor, was first introduced into the cell suspension, induction was suppressed but it was restored when actinomycin D, a RNA synthesis inhibitor, was added following anisomycin. By flow cytometry, the RNA content was slightly increased in the presence of thymosin and this increase was enhanced by the simultaneous addition of RNA and protein synthesis inhibitors.
如DNA、RNA和蛋白质合成抑制剂的作用所示，在胸腺素的影响下，脾原胸腺细胞分化为Thy-1.2+细胞需要DNA转录和RNA翻译。在存在蛋白质合成可逆抑制剂的情况下，这种T细胞分化诱导信息的寿命似乎相对较短（30至60分钟）。当蛋白质合成抑制剂茴香霉素首次引入细胞悬液时，诱导受到抑制，但在茴香霉素之后添加RNA合成抑制剂放线菌素D时，诱导恢复。通过流式细胞术，在胸腺素存在下RNA含量略有增加，同时添加RNA和蛋白质合成抑制剂可增强这种增加。
