Glasel J A, Pelosi L A
Biochem Biophys Res Commun. 1986 May 14;136(3):1177-84. doi: 10.1016/0006-291x(86)90458-4.
Polyclonal, anti-idiotypic, rabbit antibodies have been raised against four murine monoclonal anti-morphine Fab fragments. The antibody preparations, after affinity purification, have been shown to contain an anti-paratypic fraction which reversibly inhibits morphine binding to the anti-morphine antibodies and to cellular opiate receptors. Using some of the unique properties of this system, for the first time cross-reactivities of anti-paratypic antibodies with the monoclonal anti-morphine IgGs have been examined including competition for the same binding sites by a classical opiate agonist/antagonist pair.
已针对四种鼠源单克隆抗吗啡Fab片段制备了多克隆抗独特型兔抗体。经亲和纯化后的抗体制剂显示含有抗独特型组分，该组分可可逆性抑制吗啡与抗吗啡抗体以及细胞阿片受体的结合。利用该系统的一些独特性质，首次检测了抗独特型抗体与单克隆抗吗啡IgG的交叉反应性，包括经典阿片激动剂/拮抗剂对同一结合位点的竞争。
