Gresele P, Arnout J, Coene M C, Deckmyn H, Vermylen J
Biochem Biophys Res Commun. 1986 May 29;137(1):334-42. doi: 10.1016/0006-291x(86)91215-5.
A new method was developed to study leukotriene B4 (LTB4) production by stimulated whole blood. The calcium ionophore A23187 and serum-treated zymosan induced LTB4 production, measured by radioimmunoassay, in a dose- and time-dependent manner. The pattern of LTB4 production by whole blood differed markedly from that observed with isolated, purified polymorphonuclear leukocytes. Higher levels of LTB4 were reached and maintained in whole blood. The system allowed to detect drug effects on LTB4 synthesis in vitro. This new method to study the synthesis of LTB4 takes into account the complex interactions between different cell types which can modulate LTB4 metabolism.
开发了一种新方法来研究刺激全血产生白三烯B4（LTB4）的情况。钙离子载体A23187和经血清处理的酵母聚糖以剂量和时间依赖性方式诱导LTB4产生，通过放射免疫测定法进行测量。全血产生LTB4的模式与分离纯化的多形核白细胞所观察到的模式明显不同。全血中LTB4达到并维持较高水平。该系统能够检测药物对体外LTB4合成的影响。这种研究LTB4合成的新方法考虑到了不同细胞类型之间可调节LTB4代谢的复杂相互作用。
