马克斯克鲁维酵母CCT 7735中的糖转运系统。
Sugar transport systems in Kluyveromyces marxianus CCT 7735.
作者信息
da Silveira Fernando Augusto, Diniz Raphael Hermano S, Sampaio Geraldo M S, Brandão Rogelio L, da Silveira Wendel B, Castro Ieso M
机构信息
Departamento de Microbiologia Agrícola, BIOAGRO, Universidade Federal de Viçosa, Av. PH Rolfs s/n, 36571-000, Viçosa, MG, Brazil.
Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro - 35.400-000, Ouro Preto, MG, Brazil.
出版信息
Antonie Van Leeuwenhoek. 2019 Feb;112(2):211-223. doi: 10.1007/s10482-018-1143-4. Epub 2018 Aug 21.
The pattern of glucose repression in most Kluyveromyces marxianus strains does not correlate with fermentative behaviour; however, glucose repression and fermentative metabolism appear to be linked to the kinetics of sugar uptake. In this work, we show that lactose transport in K. marxianus CCT 7735 by lactose-grown cells is mediated by a low-affinity H-sugar symporter. This system is glucose repressed and able to transport galactose with low affinity. We also observed the activity of a distinct lactose transporter in response to raffinose. Regarding glucose uptake, specificities of at least three low-affinity systems rely on the carbon source available in a given growth medium. Interestingly, it was observed only one high-affinity system is able to transport both glucose and galactose. We also showed that K. marxianus CCT 7735 regulates the expression of sugar transport systems in response to glucose availability.
大多数马克斯克鲁维酵母菌株中的葡萄糖阻遏模式与发酵行为不相关；然而，葡萄糖阻遏和发酵代谢似乎与糖摄取动力学有关。在这项工作中，我们表明，乳糖培养的细胞对马克斯克鲁维酵母CCT 7735中乳糖的转运是由一种低亲和力的H-糖同向转运体介导的。该系统受葡萄糖阻遏，能够以低亲和力转运半乳糖。我们还观察到一种独特的乳糖转运体对棉子糖的响应活性。关于葡萄糖摄取，至少三种低亲和力系统的特异性取决于给定生长培养基中可用的碳源。有趣的是，仅观察到一种高亲和力系统能够转运葡萄糖和半乳糖。我们还表明，马克斯克鲁维酵母CCT 7735会根据葡萄糖的可利用性调节糖转运系统的表达。
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