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突变半乳糖 permease(_N376F)过表达有效用于利用葡萄糖/木糖或葡萄糖/半乳糖混合物的工程菌。

Overexpression of Mutant Galactose Permease (_N376F) Effective for Utilization of Glucose/Xylose or Glucose/ Galactose Mixture by Engineered .

机构信息

Department of Bioengineering and Technology, Kangwon National University, Chuncheon 24341, Republic of Korea.

Interdisciplinary Program in Biohealth-Machinery Convergence Engineering, Kangwon National University, Chuncheon 24341, Republic of Korea.

出版信息

J Microbiol Biotechnol. 2020 Dec 28;30(12):1944-1949. doi: 10.4014/jmb.2008.08035.

Abstract

Mutant sugar transporter -N376F was overexpressed in for efficient utilization of xylose, which is one of the main components of cellulosic biomass. ScGal2_N376F, the -N376F-overexpressing strain, exhibited 47.04 g/l of xylose consumption and 26.55 g/l of xylitol production, as compared to the parental strain (24.68 g/l and 7.03 g/l, respectively) when xylose was used as the sole carbon source. When a mixture of glucose and xylose was used as the carbon source, xylose consumption and xylitol production rates were improved by 195% and 360%, respectively, by ScGal2_N376F. Moreover, the glucose consumption rate was improved by 27% as compared to that in the parental strain. Overexpression of both wild-type and mutant -N376F showed 48% and 52% enhanced sugar consumption and ethanol production rates, respectively, when a mixture of glucose and galactose was used as the carbon source, which is the main component of marine biomass. As shown in this study, -N376F overexpression can be applied for the efficient production of biofuels or biochemicals from cellulosic or marine biomass.

摘要

突变型糖转运蛋白-N376F 在 中过表达,以有效利用木糖,木糖是纤维素生物质的主要成分之一。与亲本菌株(分别为 24.68 g/l 和 7.03 g/l)相比,过表达 -N376F 的 ScGal2_N376F 菌株在以木糖为唯一碳源时,表现出 47.04 g/l 的木糖消耗和 26.55 g/l 的木糖醇生产。当以葡萄糖和木糖的混合物作为碳源时,ScGal2_N376F 的木糖消耗和木糖醇生产速率分别提高了 195%和 360%。此外,与亲本菌株相比,葡萄糖消耗速率提高了 27%。野生型 和突变型 -N376F 的过表达在以葡萄糖和半乳糖的混合物作为碳源时,分别显示出 48%和 52%增强的糖消耗和乙醇生产速率,葡萄糖和半乳糖是海洋生物质的主要成分。正如本研究所示,-N376F 的过表达可应用于从纤维素或海洋生物质高效生产生物燃料或生物化学品。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4e3/9728301/87fc40cd7da5/JMB-30-12-1944-f1.jpg

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