Savabi F, Bessman S P
Biochem Med Metab Biol. 1986 Jun;35(3):345-55. doi: 10.1016/0885-4505(86)90092-7.
The functional activity, adenine nucleotides, and creatine phosphate content of spontaneously beating isolated rabbit atria were measured prior to anoxia, after 1 hr anoxia, and at the end of 1 hr reoxygenation at pH 6.7 and 7.2 During anoxia at pH 7.2 there was 13.3% loss of adenine nucleotides pool, 35.2% loss of ATP, 36.2% increase in ADP, 200% increase in AMP, and a decrease to 8.8% of CP assayed to the beating atria in oxygen. At pH 6.7 there was almost the same decrease in CP, about 10% decrease in ATP, no change in total adenine nucleotides, no change in AMP and a higher increase in ADP (88.7%). The postanoxic recovery was much more complete when the pH was 6.7 during anoxia, and the first 40 min of reoxygenation. The extent of recovery of functional activity correlated well with the level of ATP in all cases not CP. Since the adenylate kinase and ATPase activity both decrease at acidic pH, their combined diminution would tend to preserve the adenine nucleotide pool and thus the better recovery at pH 6.7, because of a decrease in energy demand and unavailability of AMP for the degradation process. This study also supports the notion of compartmented adenine nucleotides connected by the creatine phosphate-creatine energy shuttle.
在缺氧前、缺氧1小时后以及在pH 6.7和7.2条件下复氧1小时结束时，对自发搏动的离体兔心房的功能活性、腺嘌呤核苷酸和磷酸肌酸含量进行了测量。在pH 7.2缺氧期间，腺嘌呤核苷酸池损失13.3%，ATP损失35.2%，ADP增加36.2%，AMP增加200%，与在有氧条件下搏动的心房相比，CP测定值降至8.8%。在pH 6.7时，CP下降幅度几乎相同，ATP下降约10%，总腺嘌呤核苷酸无变化，AMP无变化，ADP升高幅度更大（88.7%）。当缺氧期间及复氧的前40分钟pH为6.7时，缺氧后的恢复更为完全。在所有情况下，功能活性的恢复程度与ATP水平密切相关，而非与CP相关。由于在酸性pH条件下腺苷酸激酶和ATP酶活性均降低，它们的共同降低倾向于保存腺嘌呤核苷酸池，因此在pH 6.7时恢复更好，这是因为能量需求降低以及AMP无法用于降解过程。本研究还支持由磷酸肌酸 - 肌酸能量穿梭连接的分隔腺嘌呤核苷酸的概念。
