Savabi F, Bessman S P
Arch Biochem Biophys. 1986 Jul;248(1):151-7. doi: 10.1016/0003-9861(86)90411-x.
Spontaneously beating isolated rat atria were subjected to 1 h of anoxia at 37 degrees C in various cardioplegic solutions. Contraction continued for different times upon initiation of anoxia, depending on the nature of the cardioplegic solution. Two hundred micromolar P1,P5-di(adenosine-5')pentaphosphate (Ap5A) stopped atrial function in less than 30 s of anoxia in contrast to 50 s in the case of Hearse's cardioplegic solution (16 mM MgCl2, 16 mM KCl, 1 mM Procaine), and 20 min in the case of controls. The stopping time was also prolonged from 30 to approximately 50-55 seconds if a lower concentration of Ap5A (100 microM) was used. Function, adenine nucleotides (AN), and phosphocreatine (PCr) were then measured 20 min after reoxygenation. The recovery of both function and AN was most rapid and complete with 200 microM Ap5A (97% recovery in ATP and 100% in function) and least complete in control (50% recovery in ATP and 78% in function). A positive correlation between recovery of ATP, or total adenine nucleotides, and recovery of function was observed in all cases. The higher the level of ATP remaining at the end of 1 h of anoxia and the more recovered after 20 min of reoxygenation, the more complete the recovery of function. The PCr returned to normal or even higher than normal values in all cases, even though function returned only in proportion to ATP. Since PCr is mitochondrial in origin, it appears that loss of a portion of the AN localized at the energy-utilizing sites occurred before serious mitochondrial damage and was responsible for the incomplete postanoxic functional recovery.
将自发性搏动的离体大鼠心房在37℃下于各种心脏停搏液中进行1小时缺氧处理。缺氧开始后，收缩持续不同时间，这取决于心脏停搏液的性质。200微摩尔的P1,P5-二（腺苷-5'）五磷酸（Ap5A）在缺氧不到30秒时就使心房功能停止，相比之下，Hearse心脏停搏液（16毫摩尔氯化镁、16毫摩尔氯化钾、1毫摩尔普鲁卡因）为50秒，对照组为20分钟。如果使用较低浓度的Ap5A（100微摩尔），停止时间也会从30秒延长至约50 - 55秒。复氧20分钟后测量功能、腺嘌呤核苷酸（AN）和磷酸肌酸（PCr）。使用200微摩尔Ap5A时，功能和AN的恢复最为迅速和完全（ATP恢复97%，功能恢复100%），而对照组最不完全（ATP恢复50%，功能恢复78%）。在所有情况下，均观察到ATP或总腺嘌呤核苷酸的恢复与功能恢复之间呈正相关。缺氧1小时结束时剩余的ATP水平越高，复氧20分钟后恢复得越多，功能恢复就越完全。尽管功能仅按ATP的比例恢复，但在所有情况下PCr都恢复到正常甚至高于正常值。由于PCr起源于线粒体，似乎在严重线粒体损伤之前，位于能量利用部位的一部分AN就已丢失，这导致了缺氧后功能恢复不完全。
