[Identification of Seeds and Seedlings of Chinese Medicinal Materials Using DNA Barcoding Technology:A Case Study in Paris polyphylla var. yunnanensis].

OBJECTIVE

To establish a novel approach based on DNA barcode sequence, so as to guarantee the quality stability of Chinese medicinal materials.

METHODS

Eight species of Paris plants were collected, and a standard DNA barcode library was developed by ITS loci. Furthermore, the barcodes also used to identify the seed and seedling products that purchased from the markets.

RESULTS

ITS loci can stably and accurately distinguish Paris polyphylla var. yunnanensis and its adulterants.

CONCLUSION

The seeds and seedlings of Chinese medicinal materials need to be properly authenticated before planting,and DNA barcoding has been found to be effective for this purpose.