Zhang Gai-Xia, Jin Yue, Jia Jing, Song Jing-Yuan, Shi Lin-Chun, Chen Shi-Lin
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
College of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China.
Zhongguo Zhong Yao Za Zhi. 2016 Feb;41(3):390-395. doi: 10.4268/cjcmm20160305.
In order to guarantee the species correction of Notopterygium seeds, a molecular identification method with ITS2 as DNA barcode has been verified. In this study, 27 samples of Notopterygium seeds were collected from the main producing area of Notopterygium. The morphological characteristics of the Notopterygium seeds were firstly surveyed. Then the DNA extraction, PCR amplification, DNA sequencing and DNA assembly were carried out. The species identification for a Notopterygium seed was implemented through distance method, NJ-tree method and the DNA barcoding system for traditional Chinese medicine (www.tcmbarcode.cn). The results showed that the seeds of N. incisum and N. franchetii had similar morphological characteristics and were difficult to distinguish clearly based on morphological descriptions. With the results of molecular identification, 24 samples were genuine including 13 N. incisum seeds samples and 11 N. franchetii genuine seeds samples. In conclusion, DNA barcode technology can accurately and efficiently identify the species of Notopterygium seeds. Furthermore, this study will provide a new method for germplasm resources identification of medicinal materials and supplies some guidelines for establishing Chinese herbal seeds and seedlings quality standards.
为保证羌活种子的物种准确性，已验证了一种以ITS2作为DNA条形码的分子鉴定方法。本研究从羌活主产区采集了27份羌活种子样本。首先对羌活种子的形态特征进行了调查。然后进行DNA提取、PCR扩增、DNA测序和DNA拼接。通过距离法、NJ树法以及中药材DNA条形码鉴定系统（www.tcmbarcode.cn）对羌活种子进行物种鉴定。结果表明，羌活和宽叶羌活的种子形态特征相似，基于形态描述难以清晰区分。根据分子鉴定结果，有24份样本为正品，其中包括13份羌活种子样本和11份宽叶羌活正品种子样本。综上所述，DNA条形码技术能够准确、高效地鉴定羌活种子的物种。此外，本研究将为药材种质资源鉴定提供一种新方法，并为建立中药材种子种苗质量标准提供一些指导。
