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中国药用植物变种根、茎和叶中的细菌多样性

Bacterial Diversity in Roots, Stems, and Leaves of Chinese Medicinal Plant var. .

作者信息

Liu Tian-Hao, Zhou Yin, Tao Wen-Cong, Liu Yang, Zhang Xiao-Mei, Tian Shou-Zheng

机构信息

Yunnan Key Laboratory of Molecular Biology of Chinese Medicine, Faculty of Basic Medical Science, Yunnan University of Chinese Medicine , Kunming, Yunnan , China ; College of Chinese Medicine, Jinan University , Guangzhou, Guangdong , China.

Yunnan Key Laboratory of Molecular Biology of Chinese Medicine, Faculty of Basic Medical Science, Yunnan University of Chinese Medicine , Kunming, Yunnan , China.

出版信息

Pol J Microbiol. 2020 Sep;69(1):91-97. doi: 10.33073/pjm-2020-012. Epub 2020 Mar 11.

Abstract

The root of var. , a famous and endangered traditional Chinese herb, has a significant medicinal value. The aim of this study was to analyze the composition and functional characteristics of bacterial endophytes in roots, stems, and leaves of var. . The 16S rRNA gene sequencing and functional prediction of bacterial endophytes in roots, stems, and leaves of var. were conducted. The Chao and Shannon indices of the bacteria in roots were significantly higher than those in stems and leaves. The dominant endophyte phyla were Cyanobacteria, Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The main genera detected in roots were unclassified , and ; the main genera in stems were , and ; the main genera in leaves were and . The microbiota in roots was particularly enriched in functional categories "extracellular structures" and "cytoskeleton" compared with stems and leaves ( < 0.05). Our study reveals the structural and functional characteristics of the endophytic bacteria in roots, stems, and leaves of var. , which aids in the scientific understanding of this plant. The root of var. , a famous and endangered traditional Chinese herb, has a significant medicinal value. The aim of this study was to analyze the composition and functional characteristics of bacterial endophytes in roots, stems, and leaves of var. . The 16S rRNA gene sequencing and functional prediction of bacterial endophytes in roots, stems, and leaves of var. were conducted. The Chao and Shannon indices of the bacteria in roots were significantly higher than those in stems and leaves. The dominant endophyte phyla were Cyanobacteria, Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The main genera detected in roots were unclassified , and ; the main genera in stems were , and ; the main genera in leaves were and . The microbiota in roots was particularly enriched in functional categories “extracellular structures” and “cytoskeleton” compared with stems and leaves ( < 0.05). Our study reveals the structural and functional characteristics of the endophytic bacteria in roots, stems, and leaves of var. , which aids in the scientific understanding of this plant.

摘要

[某种植物]变种是一种著名的濒危传统中草药,其根部具有重要的药用价值。本研究旨在分析[某种植物]变种的根、茎和叶中细菌内生菌的组成及功能特性。对[某种植物]变种的根、茎和叶中的细菌内生菌进行了16S rRNA基因测序和功能预测。根中细菌的Chao指数和香农指数显著高于茎和叶。优势内生菌门为蓝细菌门、变形菌门、拟杆菌门、厚壁菌门和放线菌门。根中检测到的主要属为未分类的[属名1]、[属名2];茎中的主要属为[属名3]、[属名4];叶中的主要属为[属名5]、[属名6]。与茎和叶相比,根中的微生物群在“细胞外结构”和“细胞骨架”功能类别中特别富集(P<0.05)。我们的研究揭示了[某种植物]变种根、茎和叶中内生细菌的结构和功能特征,这有助于对这种植物的科学理解。[某种植物]变种是一种著名的濒危传统中草药,其根部具有重要的药用价值。本研究旨在分析[某种植物]变种的根、茎和叶中细菌内生菌的组成及功能特性。对[某种植物]变种的根、茎和叶中的细菌内生菌进行了16S rRNA基因测序和功能预测。根中细菌的Chao指数和香农指数显著高于茎和叶。优势内生菌门为蓝细菌门、变形菌门、拟杆菌门、厚壁菌门和放线菌门。根中检测到的主要属为未分类的[属名1]、[属名2];茎中的主要属为[属名3]、[属名4];叶中的主要属为[属名5]、[属名6]。与茎和叶相比,根中的微生物群在“细胞外结构”和“细胞骨架”功能类别中特别富集(P<0.05)。我们的研究揭示了[某种植物]变种根、茎和叶中内生细菌的结构和功能特征,这有助于对这种植物的科学理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d044/7256839/35e2957f51d2/pjm-69-1-097-g001.jpg

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