College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, China.
Department of Chemical Sciences, Bernal Institute, University of Limerick, Castletroy, Ireland.
J Mol Recognit. 2019 Feb;32(2):e2761. doi: 10.1002/jmr.2761. Epub 2018 Aug 22.
A deep understanding of the molecular interactions of carbon nanodots with biomacromolecules is essential for wider applications of carbon nanodots both in vitro and in vivo. Herein, nitrogen and sulfur co-doped carbon dots (N,S-CDs) with a quantum yield of 16% were synthesized by a 1-step hydrothermal method. The N,S-CDs exhibited a good dispersion, with a graphite-like structure, along with the fluorescence lifetime of approximately 7.50 ns. Findings showed that the fluorescence of the N,S-CDs was effectively quenched by bovine hemoglobin as a result of the static fluorescence quenching. The mentioned quenching mechanism was investigated by the Stern-Volmer equation, temperature-dependent quenching, and fluorescence lifetime measurements. The binding constants, number of binding sites, and the binding average distance between the energy donor N,S-CDs and acceptor bovine hemoglobin were calculated as well. These findings will provide for valuable insights on the future bioapplications of N,S-CDs.
深入了解碳点与生物大分子的分子相互作用，对于碳点在体外和体内的更广泛应用至关重要。在此，通过一步水热法合成了量子产率为 16%的氮硫共掺杂碳点（N,S-CDs）。N,S-CDs 具有良好的分散性，石墨状结构，荧光寿命约为 7.50ns。研究结果表明，由于静态荧光猝灭，牛血红蛋白有效地猝灭了 N,S-CDs 的荧光。通过 Stern-Volmer 方程、温度猝灭和荧光寿命测量研究了所述猝灭机制。还计算了供体 N,S-CDs 和受体牛血红蛋白之间的结合常数、结合位点数和结合平均距离。这些发现将为 N,S-CDs 的未来生物应用提供有价值的见解。
