Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, 92 Wu Cheng Road, Taiyuan, 030006, Shanxi, China.
Anal Bioanal Chem. 2019 Oct;411(25):6687-6695. doi: 10.1007/s00216-019-02042-3. Epub 2019 Aug 12.
Fluorescence resonance energy transfer (FRET) is a kind of energy transfer mechanism depending on the distance between donor and acceptor, which exhibited potential application in biosensors. In this study, an efficient fluorescence "turn-on" strategy for the detection of glutathione (GSH) has been established based on FRET between nitrogen and sulfur dual-doped carbon dots (N,S-CDs) and gold nanoparticles (Au NPs). A novel N,S-CDs was synthesized by a one-pot hydrothermal treatment of 3-aminothiophenol, which possessed excellent fluorescence property with the maximum emission wavelength of 530 nm. Then, the as-prepared N,S-CDs served as energy donor to transfer energy to Au NPs via FRET process, resulting in fluorescence quenching of N,S-CDs. However, the fluorescence of N,S-CDs was recovered efficiently by adding GSH into the mixture solution of N,S-CDs and Au NPs. Therefore, the FRET assembly of N,S-CDs and Au NPs was used as a fluorescence probe for the "turn-on" sensing GSH with the linear range from 3.8 to 415.1 μM and the limit detection of 0.21 μM. This nanosensor platform was employed to monitor GSH in serum samples with satisfying results. Graphical abstract.
荧光共振能量转移（FRET）是一种依赖于供体和受体之间距离的能量转移机制，在生物传感器中具有潜在的应用。本研究基于氮硫双掺杂碳点（N，S-CDs）与金纳米粒子（Au NPs）之间的 FRET，建立了一种用于检测谷胱甘肽（GSH）的高效荧光“开启”策略。通过一锅水热处理 3-巯基苯胺合成了一种新型 N，S-CDs，具有优异的荧光性能，最大发射波长为 530nm。然后，将所制备的 N，S-CDs 作为能量供体通过 FRET 过程将能量转移到 Au NPs 上，导致 N，S-CDs 的荧光猝灭。然而，通过将 GSH 加入到 N，S-CDs 和 Au NPs 的混合溶液中，可以有效地恢复 N，S-CDs 的荧光。因此，N，S-CDs 和 Au NPs 的 FRET 组装被用作“开启”感测 GSH 的荧光探针，线性范围为 3.8 至 415.1 μM，检测限为 0.21 μM。该纳米传感器平台用于监测血清样品中的 GSH，结果令人满意。
