白细胞介素2在细胞溶质钙浓度升高之前增加T淋巴细胞膜流动性。

Interleukin 2 increases T lymphocyte membrane mobility before the rise in cytosolic calcium concentration.

作者信息

Utsunomiya N, Tsuboi M, Nakanishi M

出版信息

Biochemistry. 1986 May 6;25(9):2582-4. doi: 10.1021/bi00357a045.

Abstract

Using stopped-flow fluorometry with three different fluorescence probes [2-[(1-pyrenyl-butyryl)oxy]stearic acid, chlortetracycline and Quin 2], we have studied initial stage of T lymphocyte activation after interleukin 2 (IL-2) binding to a specific cell-surface receptor. After IL-2 binding to cytotoxic T lymphocyte (IL-2-dependent mouse LC7 and CTLL-2 cells), membrane mobilities of the cells increased first (4.5 +/- 0.3 s-1 for LC7 and 3.8 +/- 0.2 s-1 for CTLL-2), then calcium was released from intracellular stores into the cytoplasm (1.6 +/- 0.1 s-1 for LC7 and 2.1 +/- 0.1 s-1 for CTLL-2), and lastly, calcium was transported from the external medium into the cytoplasm (1.3 +/- 0.1 s-1 for LC7 and 1.5 +/- 0.1 s-1 for CTLL-2). The slowest process, the calcium influx from the external medium, was suppressed in the presence of a calcium channel blocking agent (verapamil). These observations give us a new information to discuss a model in T lymphocyte activation after IL-2 binding to cell-surface receptors.

摘要

我们使用停流荧光分析法，借助三种不同的荧光探针[2-[(1-芘丁酰基)氧基]硬脂酸、金霉素和喹啉2]，研究了白细胞介素2（IL-2）与特异性细胞表面受体结合后T淋巴细胞激活的初始阶段。IL-2与细胞毒性T淋巴细胞（依赖IL-2的小鼠LC7和CTLL-2细胞）结合后，细胞的膜流动性首先增加（LC7为4.5±0.3 s-1，CTLL-2为3.8±0.2 s-1），然后钙从细胞内储存库释放到细胞质中（LC7为1.6±0.1 s-1，CTLL-2为2.1±0.1 s-1），最后，钙从细胞外介质转运到细胞质中（LC7为1.3±0.1 s-1，CTLL-2为1.5±0.1 s-1）。最慢的过程，即钙从细胞外介质的内流，在存在钙通道阻滞剂（维拉帕米）的情况下受到抑制。这些观察结果为我们讨论IL-2与细胞表面受体结合后T淋巴细胞激活的模型提供了新的信息。