McMillen M A, Lewis T, Jaffe B M, Wait R B
J Surg Res. 1985 Jul;39(1):76-80. doi: 10.1016/0022-4804(85)90164-7.
Calcium is a divalent cation which acts both as a cofactor for critical protein function and as a "second signal" to trigger cytosol events after membrane depolarization. The presence of calcium is critical to T-lymphocyte function. Verapamil is a calcium channel blocker at the membrane level and may affect cytosol calcium-calmodulin as well. Verapamil effect was measured on two assays of lymphocyte proliferation (concanavalin A and phytohemagglutinin lectin and alloantigen stimulated) and on cell-mediated cytotoxicity. Verapamil inhibited proliferation at 10(-5) M doses and abrogated it at 10(-4) M. Verapamil interferes with critical calcium-mediated events in T lymphocyte proliferation and function. T-lymphocyte calcium channel blockade may be an effective immunosuppressive strategy.
钙是一种二价阳离子，它既作为关键蛋白质功能的辅助因子，又作为膜去极化后触发胞质溶胶事件的“第二信号”。钙的存在对T淋巴细胞功能至关重要。维拉帕米是膜水平的钙通道阻滞剂，也可能影响胞质溶胶中的钙-钙调蛋白。在淋巴细胞增殖的两种检测方法（伴刀豆球蛋白A和植物血凝素凝集素以及同种异体抗原刺激）和细胞介导的细胞毒性检测中测量了维拉帕米的作用。维拉帕米在10^(-5) M剂量时抑制增殖，在10^(-4) M时消除增殖。维拉帕米干扰T淋巴细胞增殖和功能中关键的钙介导事件。T淋巴细胞钙通道阻滞可能是一种有效的免疫抑制策略。
