Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China.
Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China.
J Biomed Mater Res A. 2018 Oct;106(10):2714-2725. doi: 10.1002/jbm.a.36487. Epub 2018 Aug 21.
Poly-(lactide-co-glycolide acid) (PLGA) has been widely investigated as scaffold material for bone tissue engineering owing to its biosafety, biodegradability, and biocompatibility. However, the bioinert surface of PLGA may fail in regulating cellular behavior and directing osteointegration between the scaffold and the host tissue. In this article, oxidized chondroitin sulfate (oCS) and type I collagen (Col I) were assembled onto PLGA surface via layer by layer technique (LbL) as an adhesive coating for the attachment of inorganic minerals. The multilayer-modified PLGA scaffold was mineralized in vitro to ensure the deposition of nanohydroxyapatite (nHAP). It was found that nHAP crystals were more uniformly and firmly attached on the multilayer-modified PLGA as compared with the pure PLGA scaffold, which remarkably improved PLGA surface and mechanical properties. Additionally, in vitro biocompatibility of PLGA scaffold, in terms of bone mesenchymal stem cells (BMSCs) attachment, spreading and proliferation was greatly enhanced by nHAP coating and multilayer deposition. Furthermore, the fabricated composite scaffold also shows the ability to promote the osteogenic differentiation of BMSCs through the up-regulation of osteogenic marker genes. Thus, this novel biomimetic composite scaffold might achieve a desirable therapeutic result for bone tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2714-2725, 2018.
聚（丙交酯-共-乙交酯酸）（PLGA）因其生物安全性、生物降解性和生物相容性而被广泛研究作为骨组织工程支架材料。然而，PLGA 的生物惰性表面可能无法调节细胞行为并指导支架与宿主组织之间的骨整合。在本文中，氧化硫酸软骨素（oCS）和 I 型胶原（Col I）通过层层技术（LbL）组装到 PLGA 表面，作为无机矿物质附着的粘合涂层。体外对多层修饰的 PLGA 支架进行矿化，以确保纳米羟基磷灰石（nHAP）的沉积。结果发现，与纯 PLGA 支架相比，nHAP 晶体更均匀、更牢固地附着在多层修饰的 PLGA 上，这显著提高了 PLGA 的表面和机械性能。此外，nHAP 涂层和多层沉积极大地增强了 PLGA 支架在骨间充质干细胞（BMSCs）附着、铺展和增殖方面的体外生物相容性。此外，所制备的复合支架还通过上调成骨标志物基因显示出促进 BMSCs 成骨分化的能力。因此，这种新型仿生复合支架可能为骨组织再生实现理想的治疗效果。© 2018 年 Wiley 期刊，公司。J Biomed Mater Res Part A：106A：2714-2725，2018 年。
