母源性血吸虫病：成年子代小鼠中针对无关抗原的白细胞介素-2、白细胞介素-10和调节性T淋巴细胞
Maternal schistosomiasis: IL-2, IL-10 and regulatory T lymphocytes to unrelated antigen in adult offspring mice.
作者信息
Fernandes Erica de Souza, Lorena Virgínia Maria de Barros, Sales Iana Rafaela Fernandes, Albuquerque Mônica Camelo Pessoa de Azevedo, Gomes Yara de Miranda, Costa Vlaudia Maria Assis, Souza Valdênia Maria Oliveira de
机构信息
Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil.
Instituto Aggeu Magalhães, Laboratório de Imunologia, Fundação Oswaldo Cruz, Recife, PE, Brasil.
出版信息
Rev Soc Bras Med Trop. 2018 Jul-Aug;51(4):546-549. doi: 10.1590/0037-8682-0338-2016.
INTRODUCTION
We evaluated IL-10, IL-2 and regulatory T cells (Treg), in response to ovalbumin (OA), in offspring from schistosomotic mouse mothers.
METHODS
We used animals born (BIM) or suckled (SIM) from infected mothers; and mice born/suckled from infected (BSIM) or non-infected mothers (CONTROL). After OA+adjuvant immunization, spleen cells were cultured, with or without OA, and doubly marked for cytometry.
RESULTS
BIM showed fewer CD4+/IL-2+ and more B220+/IL-10+ cells, whereas the SIM group showed increased Treg frequency. BSIM had fewer B220+/IL-10+ and Treg cells.
CONCLUSIONS
Separately, gestation or nursing induced immunosuppressive cells in infected mothers, but improved anti-OA immunity when combined.
引言
我们评估了感染血吸虫病的小鼠母亲所生后代中，白细胞介素-10（IL-10）、白细胞介素-2（IL-2）和调节性T细胞（Treg）对卵清蛋白（OA）的反应。
方法
我们使用了由感染母亲生育（BIM）或哺乳（SIM）的动物；以及由感染（BSIM）或未感染母亲（对照）生育/哺乳的小鼠。在OA加佐剂免疫后，脾细胞在有或无OA的情况下进行培养，并进行双重标记以用于细胞计数。
结果
BIM显示CD4+/IL-2+细胞较少，B220+/IL-10+细胞较多，而SIM组显示Treg频率增加。BSIM的B220+/IL-10+细胞和Treg细胞较少。
结论
单独来看，妊娠或哺乳在感染母亲中诱导产生免疫抑制细胞，但二者结合时可改善抗OA免疫。
Zotero 插件