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感染血吸虫的小鼠的妊娠和哺乳会在成年后代中差异地改变组蛋白去乙酰化酶 (HDACs) 的表达。

Gestation and breastfeeding in schistosomotic mice differentially alters the expression of histone deacetylases (HDACs) in adult offspring.

机构信息

Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami, Recife, PE, Brasil.

Fundação Oswaldo Cruz-Fiocruz, Instituto de Pesquisas Aggeu Magalhães, Recife, PE, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2020 Feb 3;114:e190366. doi: 10.1590/0074-02760190366. eCollection 2020.

DOI:10.1590/0074-02760190366
PMID:32022099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7012583/
Abstract

BACKGROUND

Breastfeeding or gestation in schistosomotic mothers can cause long-term alterations in the immune response of offspring.

OBJECTIVES

Evaluate the expression of histone deacetylases (HDACs) (all classes), the production of cytokines by T and B lymphocytes and macrophages, and the frequency of CD4+CD25+FoxP3+-cells in adult offspring born and/or suckled by schistosomotic mothers.

METHODS

We harvested splenocytes from offspring born to (BIM), suckled by (SIM), or born to/suckled by (BSIM) schistosomotic mothers and animals from noninfected mothers (Control) at seven-weeks old and cultured them with/without Concanavalin A. HDAC expression was evaluated by real-time quantitative polymerase chain reaction (qPCR), and cytokines and membrane markers were evaluated by fluorescence-activated cell sorting (FACS).

FINDINGS

Compared to Control, BIM mice showed increased expression of HDAC9 and frequency of CD4+IL-10+-cells. The SIM group had increased expression of HDAC1, HDAC2, HDAC6, HDAC7, HDAC10, Sirt2, Sirt5, Sirt6, and Sirt7. The BSIM group only had increased HDAC10 expression. The SIM and BSIM groups exhibited decreased frequencies of CD4+IL-4+-cells and CD4+CD25+FoxP3+-cells, along with a higher frequency of CD14+IL-10+-cells and an increase in CD45R/B220+IL-10+-cells. The BSIM group also showed a high frequency of CD4+IL10+-cells.

MAIN CONCLUSIONS

Breastfeeding induced the expression of HDACs from various classes involved in reducing inflammatory responses. However, gestation enhanced the expression of a single HDAC and breastfeeding or gestation appears to favour multiple IL-10-dependent pathways, but not cells with a regulatory phenotype.

摘要

背景

患有血吸虫病的母亲进行母乳喂养或妊娠会导致后代的免疫反应产生长期变化。

目的

评估组蛋白去乙酰化酶（HDACs）（所有类别）、T 和 B 淋巴细胞及巨噬细胞产生的细胞因子以及 CD4+CD25+FoxP3+-细胞在由患有血吸虫病的母亲分娩和/或哺乳的成年后代中的表达。

方法

我们在 7 周龄时从患有血吸虫病的母亲分娩（BIM）、哺乳（SIM）或分娩和哺乳（BSIM）的后代以及来自未感染母亲的动物（对照组）中采集脾细胞，并在有/无伴刀豆球蛋白 A（ConA）的情况下进行培养。通过实时定量聚合酶链反应（qPCR）评估 HDAC 表达，通过荧光激活细胞分选（FACS）评估细胞因子和膜标记物。

结果

与对照组相比，BIM 组小鼠的 HDAC9 表达增加，CD4+IL-10+-细胞的频率增加。SIM 组的 HDAC1、HDAC2、HDAC6、HDAC7、HDAC10、Sirt2、Sirt5、Sirt6 和 Sirt7 表达增加。BSIM 组仅 HDAC10 表达增加。SIM 和 BSIM 组的 CD4+IL-4+-细胞和 CD4+CD25+FoxP3+-细胞频率降低，CD14+IL-10+-细胞频率增加，CD45R/B220+IL-10+-细胞增加。BSIM 组还显示 CD4+IL10+-细胞频率较高。

主要结论

母乳喂养诱导了多种参与减轻炎症反应的 HDACs 的表达。然而，妊娠增强了单个 HDAC 的表达，母乳喂养或妊娠似乎有利于多种依赖 IL-10 的途径，但不有利于具有调节表型的细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/239c7a06b54a/1678-8060-mioc-114-e190366-gf7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/470a0a370a3d/1678-8060-mioc-114-e190366-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/7705a1e31d38/1678-8060-mioc-114-e190366-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/1b701e771a53/1678-8060-mioc-114-e190366-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/4b6872852cff/1678-8060-mioc-114-e190366-gf4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/06a07403b6f2/1678-8060-mioc-114-e190366-gf5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/09f56e77ad9c/1678-8060-mioc-114-e190366-gf6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/239c7a06b54a/1678-8060-mioc-114-e190366-gf7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/470a0a370a3d/1678-8060-mioc-114-e190366-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/7705a1e31d38/1678-8060-mioc-114-e190366-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/1b701e771a53/1678-8060-mioc-114-e190366-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/4b6872852cff/1678-8060-mioc-114-e190366-gf4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/06a07403b6f2/1678-8060-mioc-114-e190366-gf5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/09f56e77ad9c/1678-8060-mioc-114-e190366-gf6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/7012583/239c7a06b54a/1678-8060-mioc-114-e190366-gf7.jpg

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2
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J Biol Chem. 2019 Jan 18;294(3):827-837. doi: 10.1074/jbc.RA118.005956. Epub 2018 Nov 28.
3
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Micronutrient content and antioxidant enzyme activities in human breast milk.
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4
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