Laboratory of Neurophysiology, Department of Physiology and Pharmacology, Autonomous University of Aguascalientes, Aguascalientes, México.
Regenerative Medicine Program-Urology, Houston Methodist Research Institute, Houston, Texas.
Neurourol Urodyn. 2018 Jun;37(5):1574-1582. doi: 10.1002/nau.23505.
To evaluate the effects of a treatment with leuprolide acetate (LA) on bladder overactivity as well as the expression of gonadotropin releasing hormone receptor (GnRH-R), and neurofilaments NF68 and NF200 in female rats with overactive bladder induced by castration.
Changes in the urodynamic parameters were determined in SHAM, ovariectomized (OVX) and ovariectomized rats treated with LA (OVX-LA). A semi-quantitative analysis for the expression pattern of GnRH-R and neurofilaments NF68 and NF200 were determined.
Forty-three days after ovariectomy, rats from the OVX group have significant lower values for intercontractile interval (ICI) and compliance (C); as well as higher values for basal bladder pressure (BP) and frequency of non-voiding contractions (NVC). The systemic application of LA increased voiding volume (Vv) and pressure threshold (ThP) in the OVX-LA animals. The application of LA reduced the high frequency of NVC in the OVX rats. No significant differences were found for Vv and NVCs between the OVX-LA vs SHAM groups. At the mid part of the bladder, the presence of GnRH-R was evidenced in the urothelium of the SHAM group. The OVX animals showed different pattern of immunolabeling for GnRH-R as well as for neurofilaments NF200 and NF68, whereas in the OVX-LA group the immunofluorescence pattern was similar to the one seen in SHAM bladders (P < 0.05 for OVX vs OVX + LA).
the results suggest that systemic application of LA can improve bladder dysfunction in castrated rats, and perhaps considered as a treatment for overactive bladder conditions secondary to menopause.
评估醋酸亮丙瑞林（LA）治疗对去势诱导的膀胱过度活动症（OAB）大鼠的膀胱过度活动以及促性腺激素释放激素受体（GnRH-R）、神经丝 NF68 和 NF200 表达的影响。
在假手术（SHAM）、去卵巢（OVX）和去卵巢加 LA 治疗（OVX-LA）大鼠中确定尿动力学参数的变化。对 GnRH-R 和神经丝 NF68 和 NF200 的表达模式进行半定量分析。
去卵巢 43 天后，OVX 组大鼠的收缩间期（ICI）和顺应性（C）明显降低，基础膀胱压（BP）和非排尿收缩（NVC）频率升高。LA 的全身应用增加了 OVX-LA 动物的排尿量（Vv）和压力阈值（ThP）。LA 的应用降低了 OVX 大鼠的 NVC 高频。OVX-LA 与 SHAM 组之间 Vv 和 NVC 无显著差异。在膀胱中部，SHAM 组的尿路上皮中存在 GnRH-R。OVX 动物的 GnRH-R 免疫标记以及神经丝 NF200 和 NF68 呈现出不同的模式，而在 OVX-LA 组中，免疫荧光模式与 SHAM 膀胱相似（OVX 与 OVX+LA 相比 P<0.05）。
结果表明，LA 的全身应用可以改善去势大鼠的膀胱功能障碍，并且可以考虑作为绝经后 OAB 状况的治疗方法。
