Laboratorio de Neurofisiología, Depto. de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes Ags., Mexico.
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Mexico.
Dig Dis Sci. 2020 Feb;65(2):423-430. doi: 10.1007/s10620-019-05783-4. Epub 2019 Aug 20.
Electromyographic studies have shown that external anal sphincter activity is modified in response to distension in animals with spinal cord injury. Gonadotropin-releasing hormone and its agonist leuprolide acetate have neurotrophic properties in animals with spinal cord injury.
This study was to determine the effects of leuprolide acetate treatment on electromyographic activity of the external anal sphincter and anorectal manometry in ovariectomized rats with spinal cord injury.
Adult ovariectomized rats were divided in three groups: (a) sham of spinal cord injury, (b) spinal cord injury treated with saline solution, and (c) spinal cord injury treated with leuprolide acetate. The spinal cord injury was induced by clamping at level T9. Leuprolide acetate dosage of 10 μg/kg was proctored intramuscular for 5 weeks, commencing the day after the lesion. Electromyography of the external anal sphincter, anorectal manometry, and volume of the cecum were evaluated in all groups.
The electromyographic study of the external anal sphincter activity showed a significant improvement in injured rats treated with leuprolide acetate. Manometric analysis and cecum volume data obtained in animals with leuprolide acetate were very similar to those found in the sham group.
These results demonstrate that leuprolide acetate treatment improves the neurogenic colon in ovariectomized rats with spinal cord injury.
肌电图研究表明，脊髓损伤动物的肛门外括约肌活动会对外周扩张做出反应。促性腺激素释放激素及其激动剂亮丙瑞林在脊髓损伤动物中具有神经营养作用。
本研究旨在确定亮丙瑞林醋酸酯治疗对脊髓损伤去卵巢大鼠肛门外括约肌肌电图和直肠测压的影响。
成年去卵巢大鼠分为三组：（a）假脊髓损伤组，（b）脊髓损伤生理盐水治疗组，（c）脊髓损伤亮丙瑞林治疗组。脊髓损伤通过 T9 水平夹闭诱导。亮丙瑞林醋酸酯剂量为 10 μg/kg，肌肉内给药，伤后第 1 天开始，共 5 周。所有组均进行肛门外括约肌肌电图、直肠测压和盲肠容积评估。
肛门外括约肌活动的肌电图研究表明，亮丙瑞林治疗的损伤大鼠有显著改善。接受亮丙瑞林治疗的动物的测压分析和盲肠容积数据与假手术组非常相似。
这些结果表明，亮丙瑞林醋酸酯治疗可改善脊髓损伤去卵巢大鼠的神经源性结肠。
