福建省部分地区鼠类巴贝斯虫感染情况调查与基因鉴定

[Investigation and genetic identification on Babesia infection in rodents in some areas of Fujian Province].

作者信息

Xiao Fang-zhen, Peng Xiu-qing, Xu Guo-ying, Chen Yang, Lin Dai-hua, Deng Yan-qin

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2017 Feb;35(1):63-7.

Abstract

OBJECTIVE

To explore the status of Babesia infection in rodents and the genetic characteristics of Babesia spp. in Fujian Province.

METHODS

Rodents were captured by the night trapping method in Shaowu, Qingliu, Shunchang, Yong’an, Changle and Youxi during 2014-2015. The rodent species was identified, and information on the time and place of capture, species and sex of rodents was recorded. Blood samples was collected, in which the fragment of 18S rRNA gene of Babesia spp. was amplified by PCR. The PCR products were sequenced and the phylogenetic tree was constructed for homology analysis. Data on positive rate were analyzed with Chi-square or Fisher exact test.

RESULTS

Two hundred and nine rats were captured, comprising of 71 domestic and 138 wild rats. The overall positive rate was 9.6%(20/209). The positive rate in domestic rats was 2.8%(2/71), including one Rattus norvebicus and one Rattus flavipectus. The positive rate in wild rats was 13.0%(18/138), including 13 Bandicota indica, one Rattus losea, 2 Rattus confucianus and 2 Rattus fulvescens. The positive rate was significantly higher in wild rats than in domestic rats (P < 0.05). The Youxi region had the highest positive rate(14.9%,13/87), followed by Yong’an(13.6%, 3/22), and no positive rat was found in Qingliu. The positive rate in the male rats was 7.9%(9/114), and that in the females was 11.6%(11/95). The positive rate was highest in adult rats (10.4%,18/173), followed by young ones (6.3%,2/32). No positive rat was found in old rats. There was no significant difference in positive rate among different regions, between male and female rats, or among different ages (P > 0.05). The sequences of PCR products had a 100% homology. The BLAST results revealed the species to be Babesia microti. The phylogenetic tree showed that the sample sequence was the most homologous with Babesia microti from Zhejiang Province(GenBank Accession No: JQ609305).

CONCLUSION

There occurs Babesia microti infection in rats in part areas of Fujian Province. The positive rate was higher in wild rats than in domestic rats.

摘要

目的

探讨福建省啮齿动物巴贝斯虫感染状况及巴贝斯虫种类的遗传特征。

方法

2014年至2015年期间，采用夜捕法在邵武、清流、顺昌、永安、长乐和尤溪捕获啮齿动物。鉴定啮齿动物种类，记录捕获时间、地点、种类及性别等信息。采集血液样本，通过PCR扩增巴贝斯虫18S rRNA基因片段。对PCR产物进行测序并构建系统发育树进行同源性分析。阳性率数据采用卡方检验或Fisher确切概率法分析。

结果

共捕获209只鼠，其中家鼠71只，野鼠138只。总体阳性率为9.6%（20/209）。家鼠阳性率为2.8%（2/71），包括1只褐家鼠和1只黄胸鼠。野鼠阳性率为13.0%（18/138），包括13只板齿鼠、1只黄毛鼠、2只社鼠和2只黄腹鼠。野鼠阳性率显著高于家鼠（P<0.05）。尤溪地区阳性率最高（14.9%，13/87），其次是永安（13.6%，3/22），清流未发现阳性鼠。雄鼠阳性率为7.9%（9/114），雌鼠阳性率为11.6%（11/95）。成年鼠阳性率最高（10.4%，18/173），其次是幼鼠（6.3%，2/32），老年鼠未发现阳性。不同地区、雌雄鼠及不同年龄段之间阳性率差异均无统计学意义（P>0.05）。PCR产物序列同源性为100%。BLAST结果显示该物种为微小巴贝斯虫。系统发育树表明样本序列与来自浙江省的微小巴贝斯虫（GenBank登录号：JQ609305）同源性最高。