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(钠+钾)-ATP酶与可被氯仿:甲醇提取的一种膜成分之间的部分免疫化学同一性。

Partial immunochemical identity between (Na+ + K+)-ATPase and a membrane component extractable with chloroform: methanol.

作者信息

Addis J S, Tenbarge K M, Barrnett R J

出版信息

Cell Biochem Funct. 1985 Apr;3(2):133-8. doi: 10.1002/cbf.290030209.

DOI:10.1002/cbf.290030209
PMID:3013445
Abstract

An IgG fraction prepared from an antiserum against a holoenzyme preparation of (Na+ + K+)-ATPase precipitated a single antigen when samples of holoenzyme were subjected to crossed immunoelectrophoresis but precipitated an additional, immunochemically-related antigen when a plasma membrane-enriched fraction was subjected to crossed immunoelectrophoresis under the same conditions. The immunochemically-related antigen could be extracted from the plasma membrane fraction with CHCl3:CH3OH.

摘要

用抗(Na⁺ + K⁺)-ATP 酶全酶制剂的抗血清制备的 IgG 组分,当全酶样品进行交叉免疫电泳时沉淀出单一抗原,但在相同条件下对富含质膜的组分进行交叉免疫电泳时,沉淀出一种额外的、免疫化学相关的抗原。这种免疫化学相关抗原可用氯仿:甲醇从质膜组分中提取出来。

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Partial immunochemical identity between (Na+ + K+)-ATPase and a membrane component extractable with chloroform: methanol.(钠+钾)-ATP酶与可被氯仿:甲醇提取的一种膜成分之间的部分免疫化学同一性。
Cell Biochem Funct. 1985 Apr;3(2):133-8. doi: 10.1002/cbf.290030209.
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