Addis J S, Merrit W D, Mazurkiewicz J E, Barrnett R J
Cell Biochem Funct. 1987 Apr;5(2):135-41. doi: 10.1002/cbf.290050209.
Subcellular membrane fractions were prepared from the salt glands of osmotically-stressed ducklings. Two fractions were characterized biochemically with respect to (Na+ + K+)-ATPase, alkaline phosphodiesterase I, succinate dehydrogenase, esterase, and galactosyltransferase activities and immunochemically with respect to (Na+ + K+)-ATPase. The ratios of the estimates of the (Na+ + K+)-ATPase contents obtained biochemically and immunochemically from the two fractions differed by more than 2 X. The results are consistent with the presence of at least two molecular species of (Na+ + K+)-ATPase, unevenly distributed between the two fractions.
从渗透应激的雏鸭盐腺中制备亚细胞膜组分。对两个组分进行了关于（Na⁺ + K⁺）-ATP酶、碱性磷酸二酯酶I、琥珀酸脱氢酶、酯酶和半乳糖基转移酶活性的生化表征，以及关于（Na⁺ + K⁺）-ATP酶的免疫化学表征。从这两个组分中通过生化和免疫化学方法获得的（Na⁺ + K⁺）-ATP酶含量估计值的比率相差超过2倍。结果与至少两种分子形式的（Na⁺ + K⁺）-ATP酶的存在一致，它们在两个组分之间分布不均。
