Department of Chemistry, College of Science, UAE University, PO Box 15551, Al Ain, UAE.
Department of Biology, College of Science, UAE University, PO Box 15551, Al Ain, UAE.
BMC Complement Altern Med. 2018 Aug 22;18(1):240. doi: 10.1186/s12906-018-2285-7.
Here, we determined in vitro antioxidant activity, total phenols and flavonoids and evaluated antiproliferative activity of three medicinal plant extracts: Trigonella foenum-graecum (Fenugreek), Cassia acutifolia (Senna) and Rhazya stricta (Harmal).
The leaves of the three medicinal plants were extracted with 70% ethanol. Antioxidant activities of the extracts were determined by using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Total flavonoid and phenolic contents were determined using colorimetric assays. MTT assay was used to estimate the antiproliferative activities of the extracts against human hepatoma (HepG2) cancer cell line. In addition, the effects of R. stricta extract on cell cycle, colony formation, and wound healing of HepG2 cells and tube formation of HUVEC cells were assessed.
Percentage inhibition of DPPH scavenging activity were dose-dependent and ranged between (89.9% ± 0.51) and (28.6% ± 2.07). Phenolic contents ranged between (11.5 ± 0.013) and (9.7 ± 0.008) mg GAE/g while flavonoid content ranged between (20.8 ± 0.40) and (0.12 ± 0.0.01) mg QE/g. Antiproliferative results of the extracts were found to be consistent with their antioxidant activity. Among the extracts evaluated, that of R. stricta showed the best antioxidant, antiproliferative and antimetastatic activities at low concentration. It also inhibited the colony-formation capacity of HepG2 cells and exhibited antiangiogenic activity. Cell cycle analysis showed significant arrest of cells at G2/M phase 12 and 48 h after treatment and significant arrest at G1/S phase after 24 h of treatment. Consistent data were observed in western blot analysis of protein levels of Cdc2 and its cyclin partners.
These findings introduce R. stricta as a potentially useful anti-metastatic agent and a novel potential anti-tumour agent for hepatocellular carcinoma (HCC) treatment.
在这里，我们测定了三种药用植物提取物（葫芦巴、番泻叶和瑞香狼毒）的体外抗氧化活性、总酚和类黄酮含量，并评估了其抗增殖活性。
用 70%乙醇提取三种药用植物的叶子。使用 DPPH（1,1-二苯基-2-苦基肼）测定法测定提取物的抗氧化活性。使用比色法测定总黄酮和总酚含量。MTT 法用于评估提取物对人肝癌（HepG2）癌细胞系的抗增殖活性。此外，还评估了瑞香狼毒提取物对 HepG2 细胞的细胞周期、集落形成和伤口愈合以及 HUVEC 细胞的管形成的影响。
DPPH 清除活性的抑制率呈剂量依赖性，范围在（89.9%±0.51）和（28.6%±2.07）之间。总酚含量范围在（11.5±0.013）和（9.7±0.008）mg GAE/g 之间，而总黄酮含量范围在（20.8±0.40）和（0.12±0.0001）mg QE/g 之间。提取物的抗增殖结果与它们的抗氧化活性一致。在所评估的提取物中，瑞香狼毒提取物在低浓度下表现出最好的抗氧化、抗增殖和抗转移活性。它还抑制 HepG2 细胞的集落形成能力，并表现出抗血管生成活性。细胞周期分析显示，处理后 12 和 48 小时细胞明显停滞在 G2/M 期，处理后 24 小时细胞明显停滞在 G1/S 期。Western blot 分析细胞周期蛋白依赖性激酶 1（CDK1）及其细胞周期蛋白伙伴蛋白的水平也得到了一致的数据。
这些发现表明瑞香狼毒可能是一种有用的抗转移剂，也是治疗肝细胞癌（HCC）的一种新型潜在抗肿瘤剂。
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